丹皮酚对心肌纤维化模型大鼠的干预效果及作用机制  

Intervention effect and mechanism of paeonol on myocardial fibrosis model rats

作　　者：谭琦[1] 周小明[1] 邹瑾[2] TAN Qi;ZHOU Xiaoming;ZOU Jin(Department of Cardiology,The First Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410000,China;Hunan Vmational College of Stiente and Technology,Changsha 110004,China)

机构地区：[1]湖南中医药大学第一附属医院心内科,湖南长沙410000 [2]湖南科技职业学院,湖南长沙410004

出　　处：《西部医学》2020年第3期334-338,共5页Medical Journal of West China

摘　　要：目的探讨丹皮酚对心肌纤维化模型大鼠的干预效果及作用机制。方法选取60只雄性SPF级SD大鼠,其中50只用于构建心肌纤维化大鼠模型,40只成功建立心肌纤维化模型,随机分为模型组、低剂量丹皮酚组(10mg·kg^-1)、中剂量丹皮酚组(15mg·kg^-1)、高剂量丹皮酚组(20mg·kg^-1)各10只,另选取正常组10只。检测大鼠左心室舒张末期压力(LVEDP)、左心室收缩压(LVSP)、左心指数;观察心肌组织病理改变;计算心肌胶原容积分数(CVF);酶联免疫吸附试验检测超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢酶(CAT)、乳酸脱氢酶(LDH)、天门冬氨酸氨基转移酶(AST)、肌氨酸酐致活酶MB(CK-MB)水平;Western blot检测TGF-β1/Smad3通路相关蛋白。结果低、中、高剂量丹皮酚组大鼠左心指数、CVF、MDA、LDH、AST、CK-MB低于模型组,LVEDP、LVSP、SOD、CAT水平高于模型组(P<0.05)。高剂量丹皮酚组大鼠左心指数、CVF、MDA、LDH、AST、CK-MB低于低、中剂量丹皮酚组,LVEDP、LVSP、SOD、CAT水平高于低、中剂量丹皮酚组(P<0.05)。低、中、高剂量丹皮酚组大鼠TGF-β1、Smad2、Smad3蛋白水平低于模型组(P<0.05)。高剂量丹皮酚组TGF-β1、Smad2、Smad3蛋白水平低于低、中剂量组(P<0.05)。结论丹皮酚能够改善大鼠心功能,通过激活TGF-β-1/Smad3通路调控氧化应激相关因子水平,从而抑制心肌纤维化发展。Objective To study the intervention effect and mechanism of paeonol on myocardial fibrosis model rats.Methods By establishing myocardial fibrosis model,10 rats were randomly divided into model group,low dose paeonol group(10 mg kg^-1),middle dose paeonol group(15 mg kg^-1),high dose paeonol group(20 mg kg^-1),and 10 rats in normal group.The left ventricular end-diastolic pressure(LVEDP),left ventricular systolic pressure(LVSP)and left ventricular index were measured.The pathological changes of myocardium were observed.Collagen volume fraction(CVF)and enzyme-linked immunosorbent assay(ELISA)were calculated.Superoxide dismutase(SOD),malondialdehyde(MDA),catalase(CAT),lactate dehydrogenase(LDH),aspartate aminotransferase(AST),creatinine inactivating enzyme MB(creat MB)were detected by the attached test.Western blot was used to detect TGF-β-1/Smad3 pathway-related proteins.Results The left ventricular index,CVF,MDA,LDH,AST and CK-MB in low,medium and high dose paeonol group were lower than those in model group,while the levels of LVEDP,LVSP,SOD and CAT were higher than those in model group(P<0.05).The left ventricular index,CVF,MDA,LDH,AST and CK-MB in high-dose paeonol group were lower than those in low-dose paeonol group and middledose paeonol group,while the levels of LVEDP,LVSP,SOD and CAT were higher than those in low-dose paeonol group and middle-dose paeonol group(P<0.05).The expression of TGF-β1,Smad2 and Smad3 in low,medium and high dose paeonol group were lower than that in model group(P<0.05).The expression of TGF-β1,Smad2 and Smad3 in high-dose paeonol group were lower than that in low-dose and middle-dose paeonol group(P<0.05).Conclusion Paeonol can improve the cardiac function of rats by activating the TGF-β-1/Smad3 pathway,regulate the levels of oxidative stress-related factors,and inhibite the development of myocardial fibrosis.

关 键 词：丹皮酚 心功能 氧化应激 心肌纤维化 TGF-β1/Smad3通路 

分 类 号：R-3[医药卫生] R541