复方当归注射液对肌成纤维细胞bcl-2和bax的表达影响  

The effect of compound angelica injection on expression of bcl-2 and bax of myofibroblast

作　　者：王达 闵倩 章乐怡 WANG Da;MIN Qian;ZHANG Leyi(The First Hospital of Changsha,Changsha 410000,China;Changsha Stomatology Hospital,Changsha 410000,China)

机构地区：[1]长沙市第一医院口腔科,湖南长沙410000 [2]长沙市口腔医院,湖南长沙410000

出　　处：《西部医学》2020年第3期361-364,共4页Medical Journal of West China

摘　　要：目的探讨复方当归注射液对肌成纤维细胞bcl-2和bax表达影响及治疗口腔黏膜下纤维性变的可能性。方法体外培养人口腔黏膜成纤维细胞,并使其转分化肌成纤维细胞(MFB),分为对照组和5、10、20、40 mg/mL组5组。采用western blot法检测不同时间5组bcl-2,bax蛋白水平。结果5~40 mg/mL的复方当归注射液作用于肌成纤维细胞24、48、72 h后bax表达随时间逐渐增加,bcl-2表达则逐渐减少(P<0.05);各浓度组与对照组相比及各浓度组bcl-2和bax相比差异均具有统计学意义(P<0.01)。结论复方当归注射液可能通过影响bcl-2和bax的表达来促进MFB的凋亡,从而具有治疗口腔黏膜下纤维性变的可能性。Objective To investigate the effect of compound angelica injection on expression of Bcl-2 and Bax of myofibroblast and provide the theoretical basis for the treatment of oral submucous fibrosis.Methods Human oral mucosa fibroblasts were cultured in vitro and transformed into myofibroblasts(MFB),which were divided into control group and 5,10,20,40 mg/ml group.Western blot was used to detect the levels of bcl 2 and Bax protein in five groups at different times.Results After 5-40 mg/mL compound angelica injection on myofibroblasts for 24,48 and 72 hours,the expression of Bax increased gradually with time,while the expression of Bcl-2 decreased gradually(P<0.05).The difference of bcl 2 and Bax in each concentration group was statistically significant(P<0.01).Conclusion Compound angelica injection may promote the apoptosis of MFB by affecting the expression of bcl 2 and Bax,so it has the possibility of treating oral submucous fibrosis.

关 键 词：复方当归注射液 肌成纤维细胞 BCL-2 BAX 

分 类 号：R78[医药卫生—口腔医学]