HBV相关肝细胞癌患者中LAIR-1的表达变化及与T细胞亚群和IL-10的关系  

Expression of soluble LAIR-1 in patients with HBV related HCC and its relationship with T cell subsets and IL-10

作　　者：薛国辉[1] 孙亭立[2] 王琴[3] 淦勤 陈雪礼[1] XUE Guohui;SUN Tingli;WANG Qin(Department of Clinical Laboratory,the NO.1 People's Hospital of Jiujiang,Jiujiang,China 332000;Department of hepatobiliary surgery,the NO.1 People's Hospital of Jiujiang,Jiujiang 332000,China;Center ofphysical examination,the NO.1 People's Hospital of Jiujiang,Jiujiang 332000,China)

机构地区：[1]江西省九江市第一人民医院检验科,九江332000 [2]江西省九江市第一人民医院肝胆外科,九江332000 [3]江西省九江市第一人民医院体检中心,九江332000

出　　处：《江西医药》2020年第3期266-269,共4页Jiangxi Medical Journal

基　　金：江西省九江市科技计划项目支撑,编号201819011。

摘　　要：目的探究血清可溶性白细胞相关免疫球蛋白样受体1(Soluble leukocyte-associated immunoglobulin-like receptor-1,sLAIR-1)在HBV相关肝细胞癌(Hepatocellular carcinoma,HCC)患者中的表达变化及其与患者中的含量变化及其与T细胞亚群、IL-10和临床病理学参数间的关系。方法选取31例HBV相关HCC患者为研究对象,同时选取25例慢性乙型肝炎(chronic hepatitis B,CHB)患者和同期健康体检者(healthy control,HC)作为对照组,3组人群年龄和性别构成比无显著差异。酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)测定血清sLAIR-1和IL-10;流式细胞术检测外周血T细胞亚群频率。结果与CHB和HC组相比,HCC患者血清sLAIR-1和IL-10均显著上调,CD3+CD4+T和CD4+T/CD8+T则显著下降,而CD3+CD8+T在3组间无显著差异。血清sLAIR-1水平与HCC患者年龄、性别和肿瘤大小无相关性,而淋巴结转移、肝硬化以及TNM分期密切相关。相关性分析发现HCC患者血清sLAIR-1与IL-10呈显著正相关性。结论血清sLAIR-1在HBV相关HCC中异常上调,且与淋巴结转移、肝硬化及TNM分期相关,并与免疫抑制因子IL-10呈显著正相关性临床病理学参数及IL-10相关,可能参与了HCC的发生发展过程。Objective The aim of our study was to investigate the expression of serum soluble leukocyte associated immunoglobulin like receptor-1(sLAIR-1)in patients with HBV related hepatocellular carcinoma(HCC)and its relationship with T cell subsets,IL-10 and clinicopathological parameters.At the same period,25 patients with chronic hepatitis B(CHB)and 25 health controls(HC)were selected as the control groups.There were no significant differences in age and gender composition among the three groups.The sera levels of sLAIR-1 and IL-10 were measured by enzyme-linked immunosorbent assay(ELISA)and the frequencies of T cell subsets were measured by flow cytometry.Results When compared with CHB and HC groups,sera levels of sLAIR-1 and IL-10 in patients with HCC were significantly up-regulated,and CD3+CD4+T and CD4+T/CD8+T were significantly down regulated,while CD3+CD8+T had no significant difference among the three groups.There was no difference between groups divided according by different age,gender or tumor size of HCC patients,while significant differences were observed in different groups divided by lymph node metastasis,cirrhosis or TNM stage.The correlation analysis showed that sera levels of sLAIR-1 in patients with HCC were positively correlated with IL-10.Conclusion Sera sLAIR-1 was up-regulated in HBV related HCC,and was related with lymph node metastasis,cirrhosis and TNM stage,and was positively correlated with immunosuppressive factor IL-10.

关 键 词：肝细胞癌 可溶性白细胞相关免疫球蛋白样受体1 白介素10 T细胞亚群 

分 类 号：R735.7[医药卫生—肿瘤]