鞣花酸对皮肤基底细胞癌A431和HSC-2细胞增殖、凋亡、侵袭能力的影响  

Effects of ellagic acid on proliferation,apoptosis and invasion of human basal cell carcinoma A431 and HSC-2

作　　者：王丹 王悦欣 汪熠 李连保 WANG Dan#;WANG Yuexin;WANG Yi;LI Lianbao(Biomedical Research Center,the First People’s Hospital of Kunming,Kunming 650000,Yunnan,China;Department of Gastrointestinal Surgery,the First Affiliated Hospital of Kunming Medical University,Kunming 650000,Yunnan,China)

机构地区：[1]昆明医科大学附属甘美医院/昆明市第一人民医院生物医学实验中心,昆明650000 [2]昆明医科大学第一附属医院胃肠外科,昆明650000

出　　处：《癌症进展》2020年第4期356-358,432,共4页Oncology Progress

摘　　要：目的探讨鞣花酸对皮肤基底细胞癌A431和HSC-2细胞增殖、凋亡、侵袭能力的影响。方法选择表皮癌细胞系A431和皮肤鳞状细胞癌细胞HSC-2,应用10μg/ml的鞣花酸干预24 h,采用噻唑蓝(MTT)法检测细胞增殖,膜连蛋白V(Annexin V)-异硫氰酸荧光素(FITC)/碘化丙啶(PI)流式细胞术检测细胞凋亡,Transwell小室实验检测细胞侵袭。结果10μg/ml鞣花酸可显著抑制A431和HSC-2细胞增殖,干预24 h后,A431细胞抑制率为(28.48±0.46)%,HSC-2细胞抑制率为(21.47±1.02)%。鞣花酸干预后A431活细胞比例为(63.21±4.35)%,明显低于干预前的(90.37±2.35)%(P<0.01);鞣花酸干预后HSC-2活细胞比例为(79.21±2.32)%,低于干预前的(85.37±1.55)%(P<0.05)。鞣花酸干预后A431侵袭细胞个数为(23.26±7.23)/HP,明显低于干预前的(58.24±4.23)/HP(P<0.01);鞣花酸干预后HSC-2侵袭细胞个数为(22.56±2.23)/HP,明显低于干预前的(48.44±3.13)/HP(P<0.01)。结论鞣花酸不仅能够抑制A431和HSC-2细胞增殖,诱导其凋亡,还能够抑制其侵袭,有望成为治疗皮肤基底细胞癌的新药物。Objective To investigate the effects of ellagic acid on the proliferation,apoptosis and invasion of basal cell carcinoma A431 and HSC-2.Method Epidermal carcinoma cell line A431 and squamous cell carcinoma cell line HSC-2 were selected and treated with 10μg/ml ellagic acid for 24 h.Cell proliferation was detected by methylthiazolyldiphenyl-tetrazolium bromide(MTT).Fluorescein isothiocyanate(FITC)-Annexin V/propidium iodide(PI)flow cytometry was used to detect cell apoptosis.Transwell chamber assay was employed to detect cell invasion ability.Result 10μg/ml ellagic acid significantly inhibited the proliferation of A431 and HSC-2,achieving an inhibition rate in A431 cells of(28.48±0.46)%at 24 h after intervention,and in HSC-2 cells was(21.47±1.02)%.The proportion of viable A431 cells after intervention was(63.21±4.35)%,significantly lower than the(90.37±2.35)%before intervention(P<0.01);correspondingly,the proportion of viable HSC-2 cells was(79.21±2.32)%,and was also significantly lower compared to the(85.37±1.55)%before intervention(P<0.05).The number of invasive A431 cells was(23.26±7.23)/HP after intervention versus(58.24±4.23)/HP before intervention,and there was significant difference between the two groups(P<0.01);the number of invasive HSC-2 cells was(22.56±2.23)/HP after intervention compared to(48.44±3.13)/HP before intervention,with statistically significant difference noted(P<0.01).Conclusion Ellagic acid is expected to be a novel medication for the treatment of basal cell carcinoma,based on the effects of inhibiting proliferation,inducing apoptosis,and suppressing invasion on A431 and HSC-2 cells.

关 键 词：鞣花酸 A431细胞 HSC-2细胞 凋亡 侵袭 

分 类 号：R739.5[医药卫生—肿瘤]