黄芪桂枝五物汤对神经病理性疼痛大鼠脊髓CX3CR1表达的影响  

Effect of Huangqi Guizhi Wuwu Decoction on CX3CR1 expression in spinal cord of rats with neuropathic pain

作　　者：刘岩路[1] 王荣[1] 杨涛[1] 李建江[1] 张斌[1] 黄异飞[1] LIU Yanlu;WANG Rong;YANG Tao;LI Jianjiang;ZHANG Bin;HUANG Yifei(Affiliated Hospital of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830001, Xinjiang, China)

机构地区：[1]新疆医科大学附属中医医院,新疆乌鲁木齐830001

出　　处：《现代中西医结合杂志》2020年第7期723-727,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：新疆医科大学附属中医医院院内课题(ZYY201534)。

摘　　要：目的研究黄芪桂枝五物汤对神经病理性疼痛大鼠的镇痛作用及对脊髓背角和背根节中脊髓小胶质细胞趋化因子受体1(CX3CR1)表达的影响。方法将50只SD大鼠随机分为模型组、黄芪桂枝五物汤高剂量组、黄芪桂枝五物汤中剂量组、黄芪桂枝五物汤低剂量组、塞来昔布组,每组10只。所有大鼠均建立坐骨神经慢性缩窄性损伤神经病理性疼痛模型,术后模型组给予生理盐水10 mL/kg灌胃,黄芪桂枝五物汤高、中、低剂量组分别给予黄芪桂枝五物汤0.2 mL/kg、0.1 mL/kg、0.05 mL/kg灌胃,塞来昔布组给予塞来昔布30 mg/kg灌胃,均1次/d,连续14 d。分别在造模前及术后3,5,7,14 d采用热刺痛仪测定各组大鼠的热缩足反射潜伏期,用免疫组化法检测各组大鼠脊髓背角和背根节中CX3CR1表达情况,用荧光定量PCR法和Western blot法分别检测脊髓背角和背根节中CX3CR1 mRNA和蛋白的相对表达量。结果术后3 d各组大鼠热缩足反射潜伏期均较术前1 d明显缩短(P均<0.05),但模型组和塞来昔布组术后各时间段比较差异无统计学意义(P均>0.05);黄芪桂枝五物汤低、中剂量组从术后7 d开始、黄芪桂枝五物汤高剂量组从术后3 d开始热缩足反射潜伏期均明显长于模型组(P均<0.05)。免疫组化显示黄芪桂枝五物汤各剂量组脊髓背角和背根节中CX3CR1阳性细胞均显著少于模型组和塞来昔布组(P均<0.05),且黄芪桂枝五物汤高剂量组均明显少于黄芪桂枝五物汤低、中剂量组(P均<0.05),黄芪桂枝五物汤低、中剂量组间比较差异无统计学意义(P均>0.05)。模型组和塞来昔布组脊髓背角和背根节中CX3CR1 mRNA和蛋白相对表达量比较差异无统计学意义(P均>0.05);黄芪桂枝五物汤各剂量组脊髓背角和背根节中CX3CR1 mRNA和蛋白相对表达量均显著低于模型组和塞来昔布组(P均<0.05),且黄芪桂枝五物汤高剂量组均明显低于黄芪桂枝五物汤低、中剂量组(P�Objective It is to study the analgesic effect of Huangqi Guizhi Wuwu Decoction(HGWD)in the rats with neuropathic pain and its effect on the expression of spinal microglial chemokine receptor 1(CX3CR1)in spinal dorsal horn and dorsal root ganglia.Methods Fifty SD rats were randomly divided into model group,HGWD high dose group,HGWD medium dose group,HGWD low dose group,celecoxib group,10 rats in each group.The neuropathic pain models of chronic constrictive injury of the sciatic nerve were established in all the rats.The model group was given intragastric administration of 10 mL/kg normal saline after the surgery.The high,medium and low dose groups were given 0.2 mL/kg,0.1 mL/kg,0.05 mL/kg of HGWD by gavage respectively,and the celecoxib group was administered with 30 mg/kg of celecoxib by gavage,all once a day for 14 days.The thermal shrinkage reflex latency of each group of rats was measured before and after 3,5,7,and 14 days after modeling,and the expression of (CX3CR1)in spinal dorsal horn and dorsal root ganglia of each group were measured by immunohistochemistry.The relative expression of CX3CR1 mRNA and protein in the dorsal horn and dorsal root ganglia of the spinal cord were measured by fluorescent quantitative PCR and Western blot,respectively.Results The latency of heat shrink reflex in each group of rats was significantly shorter than that of 1 day before surgery(P<0.05),but there was no significant difference between the model group and the celecoxib group in each time period(All P>0.05);the latency of heat shrink reflex were longer in low and middle dose of HGWD groups started from 7 days after operation,and in the high dose of HGWD group started from 3 days after operation than those in the model group(all P<0.05).Immunohistochemistry showed that CX3CR1-positive cells in the spinal dorsal horn and dorsal root ganglia of each group of HGWD were significantly less than those in model group and celecoxib group(P<0.05),and the high-dose group of HGWD was significantly less than the low-and middle-dose g

关 键 词：黄芪桂枝五物汤 神经病理性疼痛 大鼠 脊髓小胶质细胞趋化因子受体1 

分 类 号：R-332[医药卫生]