接触镜储存盒不同处理方式对常见接触镜相关角膜炎病原菌生物被膜的清除作用  

Different contact lens case treatment methods for the elimination of pathogen biofilms associated with contact lens related microbial keratitis

作　　者：王帅 王嫱怡 李玉瑭 邓娟[2] 苏明泽 和凌媛 王璐薇 李彤[2] Wang Shuai;Wang Qiangyi;Li Yutang;Deng Juan;Su Mingze;He Lingyuan;Wang Luwei;Li Tong(Department of Laboratorial Science and Technology,School of Public Health,Peking University,Beijing 100191,China;Department of Microbiology and Infectious Disease Center,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100191,China;Department of Infectious Diseases and Clinical Microbiology,Beijing ChaoYang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]北京大学公共卫生学院卫生检验学系,100191 [2]北京大学基础医学院病原生物学系和感染病研究中心,100191 [3]现在首都医科大学附属北京朝阳医院感染和临床微生物科,100020

出　　处：《中华实验眼科杂志》2020年第3期175-180,共6页Chinese Journal Of Experimental Ophthalmology

摘　　要：目的评价接触镜相关角膜炎不同病原菌形成生物被膜的能力,并比较角膜接触镜储存盒(CLC)不同处理方式对其生物被膜的清除作用。方法通过静态生物被膜形成实验,在聚丙烯材质的CLC中37℃孵育金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌和白假丝酵母菌24 h,建立生物被膜模型。根据处理方式分为对照组、室温干燥组、护理液浸泡组、加热干燥组和浸泡加热组,采用系列稀释微量计数法测定各组生物被膜生物量,并计算生物被膜杀灭率。结果4个实验菌株均可在CLC内壁形成生物被膜。金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌和白假丝酵母菌形成生物被膜的生物量分别为(10.78±2.12)、(9.19±0.57)、(8.03±0.30)和(7.50±0.07)lg CFU/ml;其中金黄色葡萄球菌形成生物被膜的生物量明显高于其他菌株,差异均有统计学意义(均P<0.05)。加热干燥组和浸泡加热组各菌株生物被膜生物量均低于相应对照组,护理液浸泡组金黄色葡萄球菌和大肠埃希菌生物被膜生物量低于相应对照组,差异均有统计学意义(均P<0.05)。加热干燥处理对金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌和白假丝酵母菌的杀灭率分别为(51.76±16.75)%、(68.63±4.43)%、(83.51±13.97)%和(97.13±5.19)%,总体比较差异有统计学意义(F=31.806,P<0.001);各菌杀灭率两两比较,差异均有统计学意义(均P<0.05)。室温干燥处理对各病原菌生物被膜的杀灭率总体比较差异无统计学意义(F=0.620,P=0.606)。浸泡加热处理对大肠埃希菌和白假丝酵母菌的杀灭率分别为(100.00±0.00)%和(97.79±7.67)%,均明显高于其对金黄色葡萄球菌的(81.13±14.86)%和对铜绿假单胞菌的(74.22±11.91)%,差异均有统计学意义(均P<0.05)。结论加热处理或者结合使用护理液浸泡处理CLC可显著提高对实验菌株生物被膜菌的杀灭作用,有效清除CLC中的细菌或真菌污染。Objective To investigate the biofilm-forming abilities of pathogens associated with contact lens related microbial keratitis and to compare the efficacies of different treatments in eliminating biofilms in contact lens cases(CLCs).Methods Staphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli and Candida albicans bio lm formation in polypropylene CLCs was examined by using a static biofilm formation model,which was incubated at 37℃for 24 hours.According to the CLC treatment methods,the experimental groups were divided into a control group,a room temperature air-drying group,a contact lens care solution soaking group,a heat-drying group and a soaking-heating group.A pathogen colony counting-based serial dilution micro-counting method was applied to evaluate the biofilm elimination efficacies and pathogen killing rates of treatments.Results All four tested strains formed biofilms on the inner walls of the CLC,and the biomasses of S.aureus,P.aeruginosa,E.coli and C.albicans biofilm were(10.78±2.12),(9.19±0.57),(8.03±0.30),and(7.50±0.07)lg CFU/ml,respectively.The S.aureus biofilm biomass was significantly higher than those of the other strains(P<0.05).The biofilm biomasses of all the tested strains in the heat-drying and the soaking-heating groups were significantly lower than those in the control group(all at P<0.05);and the biofilm biomasses of S.aureus and E.coli in the soaking group were significantly lower than that in the control group(all at P<0.05).The heat-drying treatment resulted in a killing rate of(51.76±16.75)%for S.aureus,(68.63±4.43)%for P.aeruginosa,(83.51±13.97)%for E.coli,and(97.13±5.19)%for C.albicans,respectively(F=31.806,P<0.001).Significant differences were observed between the killing rates for each bacterium(all at P<0.05).The E.coli and C.albicans killing rates of the soak-heating treatment were(100.00±0.00)%and(97.79±7.67)%,respectively,and were significantly higher than(81.13±14.86)%of S.aureus and(74.22±11.91)%of P.aeruginosa(all at P<0.05).Conclusions Heating alone o

关 键 词：感染性角膜炎 角膜接触镜储存盒 生物被膜 加热 

分 类 号：R77[医药卫生—眼科]