蛇床子素对肺癌H1299细胞增殖和凋亡的作用  

Effects of osthole on the proliferation and apoptosis of human lung cancer H1299 cells and its mechanisms

作　　者：彭建明[1] 朱扣柱 叶记林[1] 苏兰娣 戴友爱[3] PENG Jian-ming;ZHU Kou-zhu;YE Ji-lin;SU Lan-di;DAI You-ai(Medical College of Yangzhou Polytechnic College,Yangzhou 225009,China;Department of Clinical Pharmacy,Wuxi Children’s Hospital;Clinical Research Center,Wuxi People’s Hospital Affiliated to Nanjing Medical University)

机构地区：[1]扬州市职业大学医学院,225009 [2]无锡市儿童医院临床药学室 [3]南京医科大学附属无锡市人民医院临床研究中心

出　　处：《天津医药》2020年第2期87-90,共4页Tianjin Medical Journal

基　　金：江苏省卫计委卫生职业技术教育科研资助项目(NJ201708);扬州市职业大学校级科研项目(2018ZR29);扬州市职业大学优秀教学团队项目资助。

摘　　要：目的探讨蛇床子素对人肺癌H1299细胞增殖、凋亡的作用及可能的机制,为临床治疗提供理论依据。方法以不同浓度蛇床子素(0、20、40、60、80、100、120、140和160μmol/L)处理H1299细胞48 h后,采用MTT法检测细胞增殖活性,以确定后续实验浓度。经不同浓度蛇床子素(0、40、80、120和160μmol/L)处理体外培养的肺癌H1299细胞,流式细胞仪检测细胞凋亡的变化,Western blot法检测B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、核因子κB(NF-κB)和磷酸化NF-κB(pNF-κB)的表达。结果与溶剂对照组(0μmol/L)比较,40、80、120、160μmol/L浓度的蛇床子素均可明显抑制人肺癌H1299细胞增殖(P<0.05)。80、120和160μmol/L蛇床子素处理H1299细胞48 h后可以明显地诱导细胞凋亡,降低BCL-2和pNF-κB的表达,升高Bax蛋白的表达(P<0.05),但对NF-κB的表达无显著影响(P>0.05)。结论蛇床子素可抑制人肺癌H1299细胞增殖并促进细胞凋亡,其机制可能与促进激活促凋亡因子Bax的表达、抑制抗凋亡因子Bcl-2的表达以及下调pNF-κB的表达有关。Objective To investigate the effect and possible mechanism of osthole on proliferation and apoptosis of human lung cancer H1299 cells,and to provide theoretical reference for its clinic treatment.Methods The H1299 cells were incubated with various concentrations of osthole(0,20,40,60,80,100,120,140 and 160μmol/L)for 48 h.The cell proliferation was examined by MTT assay to determine the dosages in subsequent experiments.With the different concentrations of osthole(0,40,80,120,and 160μmol/L)for 48 h,the cell apoptosis rate was measured by flow cytometry.The B-cell lymphoma-2(Bcl-2),Bcl-2 associated X(Bax),nuclear factor kappa-B(NF-κB)and phosphorylated NF-κB(pNF-κB)levels were determined by Western-blot assay.Results In comparison with the control group,osthole with concentrations of 40,60,80,100,120,140 and 160μmol/L could obviously inhibit the proliferation in H1299 cells(P<0.05).Osthole(80,120 and 160μmol/L)accelerated the cellular apoptosis,decreased the expressions of Bcl-2 and pNF-κB and increased the expression of Bax(P<0.05),while osthole showed no significant effect on the expression of NF-κB(P>0.05).Conclusion Osthole can inhibit H1299 cell proliferation and accelerate the cell apoptosis,which might be associated with the activation of the apoptotic factor Bax,the suppression of the expression of the anti-apoptosis factor Bcl-2 and the down-regulation of pNF-κB expression.

关 键 词：肺肿瘤 蛇床子素 细胞凋亡 NF-ΚB 

分 类 号：R734.2[医药卫生—肿瘤]