敲低NEDD9基因表达对胰腺癌BxPC-3细胞体内外功能的影响  

The effect of knockdown NEDD9 gene expression on functions of pancreatic cancer BxPC-3 cells in vitro and in vivo

作　　者：薛丽莉 吴铁龙 戴圆圆 王文远 盛颖玥 薛育政 XUE Li-li;WU Tie-long;DAI Yuan-yuan;WANG Wen-yuan;SHENG Ying-yue;XUE Yu-zheng(Department of Internal Medicine,the Affiliated Wuxi Maternity and Child Health Care Hospital of Nanjing Medical University,Wuxi 214002,China;Department of Gastroenterology,the Affiliated Hospital of Jiangnan University(Wuxi Third People's Hospital)

机构地区：[1]南京医科大学附属无锡市妇幼保健院内科,214002 [2]江南大学附属医院消化内科(无锡市第三人民医院)

出　　处：《天津医药》2020年第2期96-100,I0002,共6页Tianjin Medical Journal

基　　金：无锡市卫生计生委“科教强卫”重点人才项目(ZDRC039);无锡市卫生计生委“科教强卫”(QNRC096);无锡市卫生计生委青年科研项目(Q201747)。

摘　　要：目的探讨敲低NEDD9基因表达对胰腺癌BxPC-3细胞体内外功能的影响。方法采用NEDD9 shRNA干扰质粒(Y2602)敲低NEDD9表达,将预处理的BxPC-3细胞分为BxPC-3 Y2602组[转染干扰序列(Y2602)]、BxPC-3 NC组(不做任何处理)及BxPC-3 Blank组[转染无义序列(Y007)],通过平板克隆形成实验检测3组细胞增殖情况,划痕实验及Transwell实验检测细胞迁移及侵袭能力,同时制备3组细胞悬液接种于裸鼠皮下成瘤建立动物模型,通过比较3组裸鼠肿瘤生长情况及瘤体大小分析敲低NEDD9后对BxPC-3细胞体内功能的影响。结果与其他2组对比,BxPC-3 Y2602组细胞增殖受到抑制、迁移至小室下室面的细胞数减少、迁移距离缩短(P<0.05)。BxPC-3 Y2602组多时点检测条件下肿瘤的体积更小,瘤体质量更小(P<0.05)。结论敲低NEDD9基因表达抑制BxPC-3细胞体内外增殖、迁移及侵袭能力,其是潜在的胰腺癌治疗靶点。Objective To investigate the effect of knockdown NEDD9 gene expression on functions of pancreatic cancer BxPC-3 cells in vitro and in vivo.Methods shRNA interference plasmid(Y2602)was used to inhibit the expression of NEDD9.BxPC-3 cells were divided into BxPC-3 Y2602 group(transfected Y2602),BxPC-3 NC group(without transfected)and BxPC-3 blank group[transfected nonsense sequence(Y007)].The proliferation,cell migration and invasion ability of three groups were detected by plate cloning assay,scratch test and Transwell test,respectively.The cell suspension was prepared for nude mouse models.The tumor formation was compared between three groups.The effect of knockdown NEDD9 gene expression on functions of BxPC-3 was analyzed by comparing tumor growth and size.Results Comparing with other groups,it was found that the cell proliferation was significantly inhibited,the number of cells that migrated to the lower chamber was significantly reduced,the volume and weight of tumors were significantly lower in BxPC-3 Y2602 group(P<0.05).Conclusion The knockdown of NEDD9 gene expression can inhibit the proliferation,migration and invasion of BxPC-3 cells in vitro and in vivo,which is a potential therapeutic target for pancreatic cancer.

关 键 词：胰腺肿瘤 基因表达调控 肿瘤 NEDD9 BXPC-3细胞 Y2602 皮下移植瘤 

分 类 号：R735.9[医药卫生—肿瘤]