不同介质对真姬菇菌种保藏效果的对比  

Effect Comparison of Different Preservation Media for Strains of Hypsizygus marmoreus

作　　者：朱华玲 班立桐 黄亮 王玉 杨红澎 孙宁 ZHU Hua-ling;BAN Li-tong;HUANG Liang;WANG Yu;YANG Hong-peng;SUN Ning(College of Basic Science,Tianjin Agricultural College,Tianjin 300384,China;College of Agriculture and Resources and Environment,Tianjin Agricultural College,Tianjin 300384,China)

机构地区：[1]天津农学院基础科学学院,天津300384 [2]天津农学院农学与资源环境学院,天津300384

出　　处：《中国食用菌》2020年第2期39-42,共4页Edible Fungi of China

基　　金：天津市蔬菜产业技术体系创新团队项目(ITTVRS2017015);天津市特支计划-青年拔尖人才项目(TJTZJH-QNBJRC-2-12);天津市农业科技成果转化项目(17YFNZNC00160)。

摘　　要：选择适宜的菌种保藏介质,是有效保障真姬菇菌种品质的重要一环,不仅关系到真姬菇栽培者的直接利益,也关系到种质资源的保存与延续。通过测定菌丝生长速率和菌丝脱氢酶活力,对22种真姬菇菌种保藏介质的保藏效果进行了对比分析。结果表明0.1%的PEG6000溶液对真姬菇菌种保藏6个月和9个月的整体保藏效果最好,其次是0.1%的PEG10000溶液与超纯水,再次是0.8%葡萄糖溶液和矿泉水。0.6%葡萄糖溶液仅适于真姬菇菌种9个月的保藏,纯净水仅适于真姬菇菌种6个月的保藏。其他介质的保藏效果略差。Selection of suitable preservation media is an important part of effective preservation of Hypsizygus marmoreus strain.It is not only concerns the direct benefit of H. marmoreus cultivators, but also concerns the preservation and continuation of germplasm resources. In this paper, the preservation effects of 22 preservation media of H. marmoreus strain were compared and analyzed by measuring mycelial growth rate and mycelial dehydrogenase activity. The results showed that 0.1% PEG6000 solution had the best effect for the preservation of 6 months and 9 months, followed by 0.1% PEG10000 solution and ultrapure water, and 0.8% glucose solution and mineral water were the third. 0.6% glucose solution was only suitable for 9 months’ preservation of H. marmoreus strain, and pure water was only suitable for 6 months’ preservation. The preservation effects of other media were slightly poor.

关 键 词：保藏介质 真姬菇菌种 保藏效果 

分 类 号：S646.9[农业科学—蔬菜学]