改进中国药典方法测定苦木注射液中聚山梨酯80的含量  

Determination of Polysorbate 80 in Kumu Injection by Improved Method of Chinese Pharmacopoeia

作　　者：邓双炳 徐滢 刘明颖 廖小菁 吕武清 刘地发 DENG Shuangbing;XU Ying;LIU Mingying;LIAO Xiaojing;LV Wuqing;LIU Difa(State Key Laboratory of Innovative Natural Medicine and TCM Injections,Ganzhou,Jiangxi 341000,China;Jiangxi Qingfeng Pharmaceutical Co.Ltd,Ganzhou,Jiangxi 341000,China)

机构地区：[1]创新天然药物与中药注射剂国家重点实验室,江西赣州341000 [2]江西青峰药业有限公司,江西赣州341000

出　　处：《今日药学》2020年第1期4-6,共3页Pharmacy Today

基　　金：江西省科技计划项目(JXKJ-CXPT-2016005)。

摘　　要：目的建立苦木注射液中聚山梨酯80含量测定的可行性方法。方法采用硫氰酸钴铵比色法,制备标准曲线溶液、供试品溶液,分别在623 nm下测定吸光度,用标准曲线法计算含量。结果聚山梨酯80在0.4~1.2 mg·mL^-1浓度范围内,吸光度与浓度呈良好线性关系(r=0.9998);各浓度水平下的平均回收率为92%~105%,RSD为0.9%。结论本方法专属性强,精密、准确,可用于苦木注射液中聚山梨酯80的含量测定。OBJECTIVE To establish a feasible method for the determination of polysorbate 80 in Kumu injection.METHODS Standard curve solution and sample solution were prepared by cobalt ammonium thiocyanate colorimetry method.The absorbance was measured at 623 nm,and the content was calculated by standard curve method.RESULTS In the concentration range of 0.4-1.2 mg·mL^-1,the absorbance showed a good linear relationship with the concentration(r=0.9998).The average recovery was 92%-105%,and RSD was 0.9%.CONCLUSION The established method is specific,precise and accurate,and can be used for the determination of polysorbate 80 in Kumu injection.

关 键 词：苦木注射液 聚山梨酯80 硫氰酸钴铵比色法 含量测定 

分 类 号：A288[哲学宗教—马克思主义哲学]