S100A14蛋白在巨块型宫颈鳞癌中的表达及其与化疗疗效的相关性研究  

Expression of S100A14 protein in massive squamous cell carcinoma of the cervix and its correlation with chemotherapy efficacy

作　　者：杨惠芬[1] 周洪友[1] 张涛 赵楚楚 YANG Huifen;ZHOU Hongyou;ZHANG Tao;ZHAO Chuchu(Department of Obstetrics and Gynecology,Lishui Central Hospital in Zhejiang Province,Lishui323000,China;Department of Critical Care Unit,Lishui Central Hospital in Zhejiang Province,Lishui323000,China)

机构地区：[1]浙江省丽水市中心医院妇产科,浙江丽水323000 [2]浙江省丽水市中心医院重症医学科,浙江丽水323000

出　　处：《中国现代医生》2020年第3期25-29,38,I0002,共7页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2017KY730)。

摘　　要：目的研究S100A14蛋白在宫颈鳞癌新辅助动脉化疗前后的改变及其与化疗疗效的相关性,以期在化疗前预测宫颈鳞癌对化疗药物的敏感性,使患者得到更加有效的个体化治疗。方法选取2014年10月~2017年3月在我院因ⅠB2及ⅡA2期宫颈鳞癌行新辅助动脉化疗和宫颈癌根治术或放疗的患者40例,所有患者术前均接受以顺铂为基础的联合化疗。另取因子宫肌瘤而行子宫全切术患者的正常宫颈上皮组织35例作为对照组。采用Western blot方法、免疫组化SP法检测组织中S100A14蛋白表达及定位。分析比较S100A14蛋白在正常宫颈上皮组织和巨块型宫颈鳞癌组织中的表达差异,比较其在化疗前后巨块型宫颈鳞癌组织中的表达差异,同时分析化疗前巨块型宫颈鳞癌组织中S100A14蛋白表达与化疗疗效之间的关系。结果①化疗1~2个周期后,40例宫颈鳞癌患者中,25例为化疗有效,15例为化疗无效,总有效率为62.5%。②S100A14蛋白在巨块型宫颈鳞癌组织中的表达明显高于正常宫颈上皮组织(P<0.01)。③S100A14蛋白在化疗后巨块型宫颈鳞癌组织中的表达显著高于化疗前(P<0.05);化疗前巨块型宫颈癌组织中S100A14蛋白表达在化疗有效组中的表达低于化疗无效组(P<0.05)。结论①S100A14蛋白在宫颈鳞癌组织中高表达,在正常宫颈上皮组织中低表达。②早期巨块型宫颈鳞癌组织中S100A14蛋白表达与新辅助动脉化疗的疗效相关。③化疗前宫颈鳞癌组织中S100A14蛋白的表达情况有可能成为宫颈癌新辅助动脉化疗疗效的预测指标。Objective To study the changes of S100 A14 protein before and after neoadjuvant chemotherapy in cervical squamous cell carcinoma and its correlation with chemotherapy efficacy, in order to predict the sensitivity of cervical squamous cell carcinoma to chemotherapy before chemotherapy, so that patients can gain more effectively individualized treatment. Methods 40 patients with neoadjuvant arterial chemotherapy and radical resection of cervical cancer or radiotherapy for ⅠB2 and ⅡA2 cervical squamous cell carcinoma at our hospital from October 2014 to March 2017 were selected. All patients received cisplatin-based combined chemotherapy before surgery. In addition, 35 cases of normal cervical epithelial tissue of patients with uterine fibroids who underwent uterine hysterectomy were used as control group. The expression and localization of S100 A14 protein in tissues were detected by Western blot and immunohistochemical SP method. The differences in the expression of S100 A14 protein in normal cervical epithelial tissues and massive cervical squamous cell carcinoma were analyzed and compared. The difference in expression of S100 A14 protein in massive cervical squamous cell carcinoma before and after chemotherapy was compared. And the relationship between expression of S100 A14 protein in massive cervical squamous cell carcinoma and chemotherapy efficacy was analyzed before chemotherapy. Results(1)Of the 40 patients with cervical squamous cell carcinoma after 1 to 2 cycles of chemotherapy, 25 were effective in chemotherapy and 15 were ineffective in chemotherapy. The total effective rate was 62.5%.(2)The expression of S100 A14 protein in massive cervical squamous cell carcinoma was significantly higher than that in normal cervical epithelial tissue(P<0.01).(3)The expression of S100 A14 protein in massive cervical squamous cell carcinoma after chemotherapy was significantly higher than that before chemotherapy(P<0.05). The expression of S100 A14 protein in massive cervical cancer tissues before chemotherapy was

关 键 词：S100A14 宫颈鳞癌 新辅助动脉化疗 顺铂 敏感性 

分 类 号：R737.33[医药卫生—肿瘤]