羟基红花黄色素A通过激活Kv通道舒张大鼠冠状动脉  

Activation of myocyte voltage-gated K^+ channels is involved in HSYA-induced rat coronary arterial relaxation

作　　者：杨蓉 赵焕新 季新燕 翟晓艳 杨李旺 YANG Rong;ZHAO Huan-xin;JI Xin-yan;ZHAI Xiao-yan;YANG Li-wang(School of Basic Medical Sciences,Shanxi University of Chinese Medicine,Taiyuan 030024,China)

机构地区：[1]山西中医药大学基础医学院,山西太原030024

出　　处：《中国病理生理杂志》2020年第3期439-443,共5页Chinese Journal of Pathophysiology

基　　金：山西省中医药管理局资助项目(No.2016ZYYC13)。

摘　　要：目的:研究羟基红花黄色素A (hydroxysafflor yellow A,HSYA)对离体大鼠冠状动脉(rat coronary artery,RCA)的舒张作用,并探讨该作用与电压门控性钾通道(Kv通道)的关系。方法:采用DMT离体血管环技术明确HSYA对RCA的肌源性作用及血管内皮对该作用的影响;采用全细胞膜片钳技术观察HSYA对RCA平滑肌细胞Kv电流的影响;采用Western blot技术探讨HSYA对RCA平滑肌细胞Kv1.2和Kv1.5通道蛋白表达的影响。结果:10μmol/L和30μmol/L HSYA能够舒张60 mmol/L KCl或0.1 mmol/L U46619预收缩的RCA(P<0.05),但其对预收缩的内皮完整组和去内皮组RCA舒张作用的差异无统计学显著性(P>0.05);HSYA(3μmol/L、10μmol/L和30μmol/L)可使RCA平滑肌细胞Kv电流显著增加(P<0.05);10μmol/L和30μmol/L HSYA能够增加RCA平滑肌细胞Kv1.2和Kv1.5通道蛋白的表达。结论:HSYA可舒张预收缩的RCA,该作用与血管内皮无关,其可能的机制与增强Kv通道功能和蛋白表达有关。AIM: To observe the effects of hydroxysafflor yellow A(HSYA) on the relaxation of isolated rat coronary artery(RCA) rings and its relationship with voltage-gated K^+(Kv) channels. METHODS: The vascular tension was recorded with a wire myograph. The Kv currents of freshly isolated RCA smooth muscle cells were assessed with whole-cell patch clamp. The protein expression levels of Kv1.2 and Kv1.5 in RCA smooth muscle cells were assayed by Western blot. RESULTS: HSYA(10 μmol/L and 30 μmol/L) led to relaxation of RCA precontracted by 60 mmol/L KCl or 0.1 mmol/L U46619(P<0.05), but the effect showed no statistical difference between endothelium-intact and endothelium-denuded groups(P>0.05). HSYA(3 μmol/L, 10 μmol/L and 30 μmol/L) significantly increased the maximal Kv currents of RCA smooth muscle cells(P<0.05). HSYA(10 μmol/L and 30 μmol/L) increased the protein expression of Kv1.2 and Kv1.5(P<0.05). CONCLUSION: HSYA relaxes isolated RCA in an endothelium-independent manner. The vasodilatory effect of HSYA may be related to activation of the myocyte Kv channels.

关 键 词：羟基红花黄色素A 冠状动脉 舒张 电压门控性钾通道 

分 类 号：R363.2[医药卫生—病理学] R541.4[医药卫生—基础医学]