CD14、IL-4和TNF-α在健康人和慢性根尖周病患者组织中的免疫荧光定位  

Immunohistochemical localization of CD14, IL-4 and TNF-α in periapical tissue of healthy individuals and patients with chronic periapical diseases

作　　者：王刚 李娟 黄世光 WANG Gang;LI Juan;HUANG Shi-guang(Department of Conservative Dentistry,Affiliated Zhongshan Hospital,Sun Yat-sen University,Zhongshan 528404,China;School of Stomatology,Jinan University,Guangzhou 510632,China)

机构地区：[1]中山大学附属中山医院口腔分院牙体牙髓病科,广东中山528404 [2]暨南大学口腔医学院,广东广州510632

出　　处：《中国病理生理杂志》2020年第3期539-546,共8页Chinese Journal of Pathophysiology

基　　金：中山市社会公益科技研究专项资金立项项目(No.2018B1056)。

摘　　要：目的:观察白细胞分化抗原14(CD14)、白细胞介素4(IL-4)和肿瘤坏死因子α(TNF-α)在不同类型慢性根尖周病根尖周组织中的表达情况。方法:将88例样本分为3组:(1)正常对照组45例;(2)慢性根尖周脓肿组23例;(3)根尖周囊肿组20例。组织标本置于10%中性福尔马林固定液中浸泡48 h以上,制作连续组织切片。HE染色,于光学显微镜下观察各组的组织学变化;免疫荧光双染色,于荧光显微镜及共聚焦显微镜下分别观察TNF-α-CD14和IL-4-CD14双阳性细胞在根尖周组织中的表达。结果:(1)与正常对照组相比,两组慢性根尖周病组织中TNF-α-CD14及IL-4-CD14双阳性细胞密度均显著升高(P<0.05);(2)与根尖周囊肿组对比,慢性根尖周脓肿组织中TNF-α-CD14双阳性细胞密度显著升高(P<0.05);(3)与慢性根尖周脓肿组相比较,根尖周囊肿组IL-4-CD14双阳性细胞密度显著升高(P<0.05)。结论:TNF-α-CD14和IL-4-CD14双阳性细胞分别在慢性根尖周脓肿及根尖周囊肿中高表达。AIM: To observe the expression of cluser of differentiation 14(CD14), tumor necrosis factor-α(TNF-α) and interleukin-4(IL-4) in periapical tissues of patients with chronic periapical diseases, and to analyze the role of immunopathogenesis of CD14, TNF-α and IL-4 expression in human chronic periapical diseases. METHODS: A total of 88 samples were divided into 3 groups: healthy control group(n=45), chronic periapical abscess group(n=23), and periapical cyst group(n=20). All samples were fixed in 10% buffered formalin and stained with double immunofluorescence for identification of TNF-α-CD14 and IL-4-CD14 double-positive cells in periapical tissues. RESULTS: Compared with healthy control group, the densities of TNF-α-CD14 and IL-4-CD14 double-positive cells in the 2 groups of chronic periapical diseases were increased significantly(P<0.05). Compared with periapical cyst group, the density of TNF-α-CD14 double-positive cells in chronic periapical abscess was increased significantly(P<0.05). The density of IL-4-CD14 double-positive cells in periapical cyst group was significantly higher than that in chronic periapical abscess group(P<0.05). CONCLUSION: There is high expression of TNF-α-CD14 and IL-4-CD14 double-positive cells in the periapical tissues of the patients with chronic periapical abscess and perapical cyst, suggesting that the Th cytokines participate in the immune regulation of periapical diseases, which may be one of the immune mechanisms of the interaction between Th1 and Th2 cytokines.

关 键 词：根尖周病 肿瘤坏死因子α 白细胞介素4 白细胞分化抗原14 

分 类 号：R363.2[医药卫生—病理学] R781.4[医药卫生—基础医学]