Microbial Diversity in Rhizosphere Soil of Cotinus coggygria Based on High Throughput Sequencing  

作　　者：Zhou Jianghong Xia Fei Che Shaochen Ge Yuxuan Zhou Xiaohong 

机构地区：[1]Beijing Key Laboratory of Ecological Function Assessment and Regulation Technology of Green Space,Beijing Institute of Landscape Architecture,Beijing 100102,China [2]Management Department of Beijing Fragrant Hills Park,Beijing 100093,China [3]Beijing Florascape Co.,Ltd.,Beijing 100044,China

出　　处：《Plant Diseases and Pests》2019年第5期11-15,23,共6页植物病虫害研究（英文版）

基　　金：Supported by Science and Technology Project from the Beijing Municipal Administration Center of Parks(ZX2018016).

摘　　要：In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively,were conducted by high throughput sequencing(Illumina MiSeq).The results showed that the fungal diversity in the rhizosphere soil samples of C.coggygria in Fragrant Hills park in 2018 was significantly higher than that in 2016,and it was higher in the rhizosphere soil of healthy C.coggygria in Xunlupo than that in diseased ones in 2018.Verticillium dahliae,which is the causal agent of C.coggygria wilt,was detected in five soil samples.In 2018,the bacterial diversity in the rhizosphere soil of diseased C.coggygria in Xunlupo was the lowest,while it was the highest in the rhizosphere soil of healthy C.coggygria under Platycladus orientalis in Langfengting.In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park, the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively, were conducted by high throughput sequencing(Illumina Mi Seq). The results showed that the fungal diversity in the rhizosphere soil samples of C. coggygria in Fragrant Hills park in 2018 was significantly higher than that in 2016, and it was higher in the rhizosphere soil of healthy C. coggygria in Xunlupo than that in diseased ones in 2018. Verticillium dahliae, which is the causal agent of C. coggygria wilt, was detected in five soil samples. In 2018, the bacterial diversity in the rhizosphere soil of diseased C. coggygria in Xunlupo was the lowest, while it was the highest in the rhizosphere soil of healthy C. coggygria under Platycladus orientalis in Langfengting.

关 键 词：Cotinus coggygria VERTICILLIUM WILT RHIZOSPHERE soil MICROBIAL diversity High THROUGHPUT SEQUENCING 

分 类 号：S154.3[农业科学—土壤学]