17β-E2对SD大鼠DRG神经元GABAA受体的调节  

17β-E2 regulates GABAA receptor in DRG neurons of SD rats

作　　者：相龙全 文益杨 庞钰文 Xiang Longquan;Wen Yiyang;Pang Yuwen(Department of Pathology,Jining First People’s Hospital,Jining 272011,China)

机构地区：[1]济宁市第一人民医院病理科,272011

出　　处：《国际医药卫生导报》2020年第5期612-616,共5页International Medicine and Health Guidance News

基　　金：山东省医药卫生科教发展计划项目(2017WS146)。

摘　　要：目的观察雌激素17β-E2对SD大鼠背根神经节(Dorsal Root Ganglion,DRG)神经元γ-氨基丁酸A受体(GABAA Receptor,GABAAR)的调制作用。方法离体培养SD大鼠DRG神经元,分别利用Western blot技术和全细胞膜片钳技术检测GABAAR表达和功能的改变。结果①17β-E2处理的不同浓度组与对照组相比差异无统计学意义(P>0.05,n=5);②17β-E2处理的不同时长组和对照组相比差异无统计学意义(P>0.05,n=5);③给予蝇蕈醇(GABAAR特异性激动剂)后,91.6%(87/95)的DRG神经元上记录到内向电流,并可被荷包牡丹碱(GABAAR特异拮抗剂)所阻断;④80.8%(59/73)的GABAA电流被提前孵育的17β-E2可逆性地抑制。结论雌激素17β-E2对GABAA电流有明显的抑制作用,可能通过此途径参与痛觉调节程序。Objective To investigate the effect of estrogen 17β-E2 in regulating GABAA receptor(GABAAR)in dorsal root ganglion(DRG)neurons of SD rats.Methods DRG neurons of SD rats were cultured in vitro with cell culture techniques.The expression and function of GABAAR were detected by Western blotting and whole cell patch clamp technique.Results(1)There were no statistically significant differences between the treatment groups with different concentrations of 17β-E2 and the control group(P>0.05,n=5).(2)There were no statistically significant differences between the 17β-E2 treatment groups at different time points and the control group(P>0.05,n=5).(3)After application of GABAAR-specific agonist(muscimol),inward currents were recorded in 91.6%(87/95)of DRG neurons,which could be blocked by dicentrine,a specific antagonist of GABAAR.(4)80.8%(59/73)of GABAA currents were reversibly inhibited by preincubation of 17β-E2.Conclusion 17β-E2 significantly inhibits GABAA currents and may participate in pain adjustment procedures through this pathway.

关 键 词：雌激素 Γ-氨基丁酸A受体 背根神经节 

分 类 号：R74[医药卫生—神经病学与精神病学]