Omega-3 PUFA抑制大肠癌细胞株转移差异基因表达的筛选  

Screening of Omega-3 PUFA inhibiting differential gene expression in colorectal cancer cell lines

作　　者：刘春安 汪雄 龙朝辉[1] 熊卫锋[1] 张云庆 裴靖 罗婷 毛丽君 Liu Chun’an;Wang Xiong;Long Zhaohui;Xiong Weifeng;Zhang Yunqing;Pei Jing;Luo Ting;Mao Lijun(Ji'an Central People's Hospital,Ji'an 343000,China;Medical School,Jinggangshan University,Ji'an 343000,China)

机构地区：[1]吉安市中心人民医院,343000 [2]井冈山大学医学部,吉安343000

出　　处：《国际医药卫生导报》2020年第6期741-745,共5页International Medicine and Health Guidance News

基　　金：江西省卫生计生委科技计划项目(赣卫科教字[2016]22号)。

摘　　要：目的流行病学调查认为Omega-3多不饱和脂肪酸(Omega-3 PUFA)与大肠癌的发病率呈负相关,但具体机制仍不明确。本文研究Omega-3 PUFA抑制大肠癌转移的差异基因并通过实时PCR验证。方法采用MTT法检测Omega-3 PUFA对大肠癌细胞株活力的影响,确定最佳浓度及时间,采用Illumina公司开发的人全基因组表达谱芯片,检测差异基因,运用Real-time PCR验证候选差异基因。结果Omega-3 PUFA抑制HCT116细胞的活力作用,以30μg/ml及48 h时段最明显,OD值为0.42,抑制率为53.78%;Omega-3 PUFA抑制SW480细胞的活力作用,以30μg/ml及48 h时段最明显,OD值为0.29,抑制率为71.01%。从实验组和对照组2株大肠癌细胞中交集出61个共同差异基因,结合文献,与转移有关基因只有COL11A2(下调基因),下调倍数为4~5倍。Real-time PCR验证候选差异基因与基因芯片结果一致。结论Omega-3 PUFA抑制大肠癌的转移,与COL11A2基因有关联。Objective Epidemiological investigation showed that Omega-3 polyunsaturated fatty acid(Omega-3 PUFA)was negatively correlated with the incidence of colorectal cancer,but the specific mechanism was still unclear.The differential genes of Omega-3 PUFA inhibiting metastasis of colorectal cancer were studied and verified by real-time PCR.Methods MTT were used to detect the effect of Omega-3 PUFA on the activity of colorectal cancer cell lines.The optimal concentration and time were determined.The Human HT-12 v4 Expression Bead Chip developed by Illumina was used to detect the differential genes.Real-time PCR was used to verify the results of candidate differential genes.Results The most optimal condition that Omega-3 PUFA inhibited the vitality of HCT116 cell was 30μg/ml and 48 h,and the inhibition rate was 53.78%.The most optimal condition that Omega-3 PUFA inhibited the vitality of SW480 cell was 30μg/ml and 48 h,and the inhibition rate was 71.01%.From the experimental group and the control group,61 common differentially expressed genes were found in the two colorectal cancer cells.Combined with literature,the gene related to metastasis was COL11A2.Realtime PCR verified the results of differential expressed genes consistent with the microarray results.Conclusion Omega-3PUFA significantly inhibites metastasis of colorectal cancer,which relates to COL11A2 gene.

关 键 词：实时PCR Omega-3PUFA 大肠癌 转移 差异基因 

分 类 号：R735[医药卫生—肿瘤]