Identification of a novel effector BxSapB3 that enhances the virulence of pine wood nematode Bursaphelenchus xylophilus  

作　　者：Xin Huang Longjiao Hu Xiaoqin Wu 

机构地区：[1]Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037,China [2]Jiangsu Key Laboratory for Prevention and Management of Invasive Species,Nanjing Forestry University,Nanjing 210037,China

出　　处：《Acta Biochimica et Biophysica Sinica》2019年第10期1071-1078,共8页生物化学与生物物理学报（英文版）

基　　金：supported by the grants from the National Key Research and Development Program of China(No.2018YFD0600203);the Jiangsu Provincial Agricultural Science and Technology Innovation Fund(No.CX(16)1005);the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).

摘　　要：Pine wilt disease,caused by the pine wood nematode Bursaphelenchus xylophilus,leads to severe damage to pine forests in China.In our previous study,effectors secreted by this pathogen were shown to play roles in the different infection stages of pine w ilt disease,and a series of candidate effectors were predicted by transcriptom e sequencing.This study identified and characterized a novel effector,BxSapB3,which was among these candidate effectors.Agrobacterium-mediated transient expression was used to identify BxSapB3.BxSapB3 was secreted by B.xylop hilus and f.ound to be capable of inducing cell death in Nicotiana benthamiana.Quantitative real-time PCR(qRT-PCR)analysis revealed that BxSapB3 was upregulated in a highly virulent strain of B.xylophilus and expressed at lower levels in a weakly virulent strain at the early stages of infection.When BxSapB3 was silenced in B.xylophilus,the process of infection was delayed.These results indicate that BxSapB3 acts as an effector and contributes to virulence at the early stages of B.xylophilus infection.

关 键 词：BURSAPHELENCHUS xylophilus EFFECTOR VIRULENCE RNA INTERFERENCE PINE WILT disease BxSapB3 

分 类 号：S85[农业科学—兽医学]