不同砧穗组合对豆瓣菜扦插苗生理特性及硒积累的影响  

Effects of different rootstock combinations on physiological characteristics and selenium accumulation of Nasturtium officinale cuttings

作　　者：黄科文[1] 李克强 刘继[2] 岁立云[2] 刘磊[2] 王铤[2] 郑阳霞[1] 林立金[3] 廖明安[1] HUANG Kewen;LI Keqiang;LIU Ji;SUI Liyun;LIU Lei;WANG Ting;ZHENG Yangxia;LIN Lijin;LIAO Ming an(College of Horticulture,Sichuan Agricultural University,Chengdu 611130,China;Chengdu Academy of Agriculture and Forestry Sciences,Chengdu 611130,China;Institute of Pomology and Olericulture,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学园艺学院,四川成都611130 [2]成都市农林科学院,四川成都611130 [3]四川农业大学果蔬研究所,四川成都611130

出　　处：《浙江农业学报》2020年第3期447-454,共8页Acta Agriculturae Zhejiangensis

基　　金：四川省教育厅项目(17ZB0342)。

摘　　要：采用盆栽试验,分别以青菜、油菜、萝卜、蔊菜、青花菜为砧木,豆瓣菜为接穗,在土壤硒浓度为10 mg·kg^-1的条件下研究了不同砧木对豆瓣菜扦插苗生长、色素积累、保护酶活性和富硒能力的影响。结果表明,与未嫁接的豆瓣菜扦插苗相比,以油菜、萝卜、青花菜为砧木嫁接后可以显著提高豆瓣菜扦插苗各部位的生物量、叶绿素a、叶绿素b、类胡萝卜素的含量;以蔊菜为砧木嫁接后不仅使豆瓣菜扦插苗的生物量处于较低水平,对其SOD、POD、CAT活性的抑制作用也最明显;虽然以青菜为砧木嫁接后显著提高了豆瓣菜扦插苗的光合色素含量,及SOD、CAT活性,但其根、茎、叶的生物量均显著低于对照。另外,以青花菜为砧木的嫁接处理在显著提高豆瓣菜扦插苗CAT活性和可溶性蛋白含量的同时,有效降低了丙二醛含量,对豆瓣菜的耐硒能力具有显著的增强效果。5种砧木对豆瓣菜扦插苗硒积累的影响存在明显差异,其中以萝卜为砧木的嫁接处理使豆瓣菜扦插苗各部分的硒含量和地上部分的硒积累量达到最大,可为富硒蔬菜的栽培、选育提供参考。Pot experiment was carried out with Brassica chinensis,Brassica napus,Raphanus sativus,Rorippa dubia,Brassica oleracea as rootstocks,and Nasturtium officinale was used as the scion.The effects of different rootstocks on the growth,pigment accumulation,protective enzymes activities and selenium accumulation ability of N.officinale cuttings were studied under the conditions of soil selenium concentration of 10 mg·kg^-1.The results showed that after grafting with B.napus,R.sativus and B.oleracea,the biomass of all parts of N.officinale cuttings as well as chlorophyll a,chlorophyll b and carotenoid contents were significantly higher than un-grafted N.officinale.The grafting of R.dubia as a rootstock not only decreased the biomass of the N.officinale cuttings,but also inhibited the activity of SOD,POD and CAT.Although grafting of B.chinensis as rootstock significantly increased the photosynthetic pigment content,SOD and CAT activities of N.officinale cuttings,the biomass of roots,stems and leaves were significantly lower than those of the control.In addition,the grafting treatment with B.oleracea as a rootstock significantly improved the CAT activity and soluble protein content of the N.officinale cuttings,and effectively reduced the malondialdehyde content,and significantly enhanced the selenium-tolerant ability of N.officinale cuttings.There were significant differences in the effects of five kinds of rootstocks on selenium accumulation in N.officinale cuttings.Among them,the grafting treatment of R.sativus as rootstocks maximized the selenium content and selenium accumulation in the shoots of N.officinale cuttings,which could provide some reference for the cultivation and breeding of selenium-accumulation vegetables.

关 键 词：豆瓣菜 嫁接 扦插 硒 生长 

分 类 号：S636.9[农业科学—蔬菜学]