Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat  

作　　者：Tuo Qi Jia Guo Peng Liu Fuxin He Cuiping Wan Md Ashraful Islam Brett M.Tyler Zhensheng Kang Jun Guo 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,P.R.China [2]Center for Genome Research and Biocomputing,Oregon State University,Corvallis,OR,USA

出　　处：《Molecular Plant》2019年第12期1624-1638,共15页分子植物（英文版）

基　　金：This study was financially supported by the National Natural Science Foundation of China(31972224,31430069,and 31620103913);the National Key R&D Program of China(2018YFD0200402);the 111 Project from the Ministry of Education of China(no.B07049).

摘　　要：Puccinia striiformis f.sp.tritici(Pst),a biotrophic plant pathogen,secretes numerous effectors to modulate host defense systems.Understanding the molecular mechanisms by which Pst effectors regulate wheat immunity is of great importance for the development of novel strategies for durable control of stripe rust.In this study,we identified a glycine-serine-rich effector gene,PstGSRE1,which is highly induced dur-ing early infection.Transgenic expression of PstGSRE1 RNAi constructs in wheat significantly reduced virulence of Pst and increased H2O2 accumulation in wheat.PstGSRE1 was shown to target the reactive ox-ygen species(ROS)-associated transcription factor TaLOL2,a positive regulator of wheat immunity.PstGSRE1 disrupted nuclear localization of TaLOL2 and suppressed ROS-mediated cell death induced by TaLOL2,thus compromising host immunity.This work reveals a previously unrecognized strategy whereby rust fungi exploit the PstGSRE1 effector to defeat ROS-associated plant defense by modulating the subcellular compartment of a host immune regulator and facilitate pathogen infection.

关 键 词：host-induced gene silencing EFFECTOR glycine-serine-rich transcription factor PUCCINIA STRIIFORMIS f.sp.tritici reactive oxygen species 

分 类 号：S51[农业科学—作物学]