YR36/WKS1-Mediated Phosphorylation of PsbO,an Extrinsic Member of Photosystem Ⅱ,Inhibits Photosynthesis and Confers Stripe Rust Resistance in Wheat  

作　　者：Shuai Wang Qiu-Ping Li Jianfeng Wang Yan Yan Guo-Liang Zhang Yan Yan Huifei Zhang Jiajie Wu Feng Chen Xiaojie Wang Zhensheng Kang Jorge Dubcovsky Jin-Ying Gou 

机构地区：[1]State Key Laboratory of Genetic Engineering,MOE Key Laboratory for Biodiversity Science and Ecological Engineering,MOE Engineering Research Center of Gene Technology,Institute of Plant Biology,School of Life Sciences,Fudan University,Shanghai 200438,China [2]State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China [3]Agronomy College/National Key Laboratory of Wheat and Maize Crop Science,Henan Agricultural University,Zhengzhou,Henan 450046,China [4]State Key Laboratory of Crop Biology/College of Agronomy,Shandong Agricultural University,Tai^n,Shandong 271018,China [5]Department of Plant Sciences,University of California,Davis,CA 95616,USA [6]Howard Hughes Medical Institute,Chevy Chase,MD 20815,USA

出　　处：《Molecular Plant》2019年第12期1639-1650,共12页分子植物（英文版）

基　　金：This research is supported by the National Key R&D Program of China(2016YFD0100500);the National Science Foundation of China(NSFC)(31772146,31972350)for J.G.J.D.acknowledges support from BARD and the Howard Hughes Medical Institute.We thank an open project for support from the National Key Laboratory of Wheat and Maize Crop Science,Henan Agricultural University.

摘　　要：Wheat stripe rust,due to infection by Puccinia striiformis f.sp.tritici(Pst),is a devastating disease that causes significant global grain yield losses.Yr36,which encodes Wheat Kinase START1(WKS1),is an effec-tive high-temperature adult-plant resistance gene and confers resistance to a broad spectrum of Pst races.We previously showed that WKS1 phosphorylates the thylakoid ascorbate peroxidase protein and reduces its ability to detoxify peroxides,which may contribute to the accumulation of reactive oxygen species(ROS).WKS1-mediated Pst resistance is accompanied by leaf chlorosis in Pst-infected regions,but the un-derlying mechanisms remain elusive.Here,we show that WKS1 interacts with and phosphorylates PsbO,an extrinsic member of photosystem Ⅱ(PS Ⅱ),to reduce photosynthesis,regulate leaf chlorosis,and confer Pst resistance.A point mutation in PsbO-A1 or reduction in its transcript levels by RNA interference resulted in chlorosis and reduced Pst sporulation.Biochemical analyses revealed that WKS1 phosphorylates PsbO at two conserved amino acids involved in physical interactions with PS Ⅱ and reduces the binding affinity of PsbO with PS Ⅱ.Presumably,phosphorylated PsbO proteins dissociate from the PS Ⅱ complex and then un-dergo rapid degradation by cysteine and aspartic proteases.Taken together,these results demonstrate that perturbations of wheat PsbO by point mutation or phosphorylation by WKS1 reduce the rate of photo-synthesis and delay the growth of Pst pathogen before the induction of ROS.

关 键 词：WHEAT STRIPE rust PSBO WKS1 CHLOROSIS PHOTOSYNTHESIS rate 

分 类 号：S51[农业科学—作物学]