广东省部分奶牛场芽囊原虫感染情况及亚型分析  

Infection and subtype analysis of Blastocystis in some dairy farms in Guangdong

作　　者：王璐阳 曹乐天 王艳歌 崔朝辉[1] 梁楠[1] 张龙现[1] WANG Lu-yang;CAO Le-tian;WANG Yan-ge;CUI Zhao-hui;LIANG Nan;ZHANG Long-xian(College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zheng-zhou 450046,China)

机构地区：[1]河南农业大学牧医工程学院,河南郑州450046

出　　处：《中国兽医学报》2020年第2期345-348,共4页Chinese Journal of Veterinary Science

基　　金：“十三五”国家重点研发计划基金资助项目(2017YFD0501305);河南省自然科学基金资助项目(162300410129)。

摘　　要：芽囊原虫是一种常见的寄生于人类和多种动物肠道的人兽共患单细胞病原体,可引起人和动物的腹泻和肠痉挛等胃肠道疾病。为了解广东省部分地区奶牛芽囊原虫的感染情况及人兽共患特性,基于核糖体小亚基(SSU rRNA)基因位点对广东地区4个奶牛场的479份粪便样品进行PCR扩增。结果显示,芽囊原虫总感染率为1.88%(9/479),主要见于断奶前和断奶后犊牛,其感染率分别为3.38%(7/207)和1.61%(2/124),在育成牛和成年牛中未发现芽囊原虫感染。经种系发育分析发现在该地区共存在2种人兽共患亚型(ST3、ST5)和1种动物特异性亚型(ST10)。结果表明,广东地区奶牛芽囊原虫存在着潜在的人兽共患风险,具有重要的公共卫生学意义。Blastocystis is a common zoonotic single-cell pathogen parasitic on the intestinal of humans and a variety of animals,causing diarrhea,intestinal spasm and other gastrointestinal diseases in humans and animals.In order to understand the prevalence and zoonotic characteristics of Blastocystis in dairy cattle in Guangdong Province,a total of 479 fecal samples of dairy cattle from four dairy farms in Guangdong Province were amplified by PCR based on small ribosomal subunit(SSU rRNA) gene loci.The results showed that the prevalence rate of Blastocystis was 1.88%(9/479),the infection rates of pre-weaning and post-weaning calves were 3.38%(7/207) and 1.61%(2/124) respectively,and Blastocystis infection was not found in breeding and adult cattle.Two zoonotic subtypes(ST3 and ST5) and one animal-specific subtype(ST10) were found in this area with phylogenetic analysis.Research shows there was zoonotic potential of Blastocystis in dairy cattle in Guangdong Province,and this study had important public health significance.

关 键 词：芽囊原虫 奶牛 基因亚型 人兽共患 SSU RRNA ST3 ST5 ST10 

分 类 号：S852.71[农业科学—基础兽医学] R535[农业科学—兽医学]