隔药饼灸对子宫内膜异位症裸鼠异位内膜组织mi-RNAs表达的影响  

Effects of Herb-Partitioned Moxibustion on mi-RNAs Expression in Nude Rats with Endometriosis

作　　者：周志刚 肖小文[2] 周平生[1] 王晓洁[2] 戴颖恒 吴怡曼 徐鹏 徐华[1] 周凌云 吴翔[1] 陈琪 ZHOU Zhigang;XIAO Xiaowen;ZHOU Pingsheng;WANG Xiaojie;DAI Yinheng;WU Yiman;XU Peng;XU Hua;ZHOU Lingyun;WU Xiang;CHEN Qi(International Education College of Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,Jiangxi,China;Graduate College of Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,Jiangxi,China)

机构地区：[1]江西中医药大学国际教育学院,江西南昌330006 [2]江西中医药大学研究生院,江西南昌330004

出　　处：《中华中医药学刊》2020年第1期191-196,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：江西省自然科学基金(20171BAB205077);江西省教育厅科学技术研究项目(GJJ160808)。

摘　　要：目的探讨隔药饼灸对子宫内膜异位症(EMs)裸鼠异位内膜组织mi-RNAs表达的影响。方法将16只SPF级BALB/c-nu小鼠14只,先分为两组。即正常组4只,模型组10只,模型组采用自体移植方法进行造模,造模成功后随机分成模型组和隔药饼灸组,每组4只。在治疗14 d后,采用Trizol法提取各组异位内膜组织,质控后采用RNA-seq高通量测序方法分析各组的差异表达mi-RNAs,筛选出差异表达mi-RNAs,结合生物信息学对其靶基因进行分析。结果模型组与正常组比较有差异表达的mi-RNAs共75个,其中上调有37个,下调有38个;隔药饼灸与模型组比较有差异表达的miRNAs共14个,其中上调5个,下调9个;经GO和KEGG分析,隔药饼灸影响mi-RNAs的靶基因主要与蛋白结合及激素代谢有关。结论 mi-RNAs在正常组、EMs模型组及隔药饼灸治疗异位内膜组织后的异常表达,提示mi-RNAs在EMs发生发展及隔药饼灸治疗中起重要调控作用。Objective To investigate the effect of herb-partitioned moxibustion on mi-RNAs expression in nude rats with EMs. Methods Fourteen female BALB/c-nude rats in SPF level were divided into two groups:10 nude rats in the model group and 4 nude rats in the normal group. The model group’s rats adopted the autologous transplantation and the rats were killed to conform whether the EMs model was established successfully. And then the model group’s rats were randomly divided into the EMs-model group and the herb-partitioned moxibustion group with four in each group. After 14-day treatment, the ectopic endometrial tissues of each group were extracted by Trizol method. After quality control, differentially expressed mi-RNAs were analyzed by RNA-Seq high-throughput sequencing method, and the target genes of differentially expressed mi-RNAs were selected to be analyzed with combination of biological information. Results Compared with normal group’s rats, the model group’s rats had 75 differentially expressed mi-RNAs in ectopic endometrium tissues in total, including 37 up-regulated and 38 down-regulated mi-RNAs. There were 14 differentially expressed mi-RNAs in the herb-partitioned moxibustion group compared with model group, including 5 up-regulated and 9 down-regulated. Conclusion Analyzed by Go and KEGG, the target genes of mi-RNAs which were influenced by herb-partitioned moxibustion related with combination of protein and hormone metabolism, it may indicate that herb-partitioned moxibustion plays an important role in regulating the expression of mi-RNAs during the process of treating EMs.

关 键 词：子宫内膜异位症 MICRORNA表达谱 隔药饼灸 RNA-SEQ 

分 类 号：R245.8[医药卫生—针灸推拿学]