Arabidopsis CAMTA Transcription Factors Regulate Pipecolic Acid Biosynthesis and Priming of Immunity Genes  

作　　者：Yongsig Kim Sarah J.Gilmour Lumen Chao Sunchung Park Michael F.Thomashow 

机构地区：[1]MSU-DOE Plant Research Laboratory,Michigan State University,East Lansing,Ml 48824,USA [2]MSU Plant Resilience Institute,Michigan State University,East Lansing,Ml 48824,USA [3]Department of Plant,Soil and Microbial Sciences,Michigan State University,East Lansing,Ml 48824,USA [4]Present address:U.S.Department of Agriculture,Agricultural Research Service,1636 East Alisal St.,Salinas,CA 93905,USA

出　　处：《Molecular Plant》2020年第1期157-168,共12页分子植物（英文版）

基　　金：This work was supported by grants to M.F.T.from Michigan AgBioRe-search(M1CL02415);the MSU Foundation and infrastructure support from the Chemical Sciences,Geosciences,and Biosciences Division,Of fice of Basic Energy Sciences,US Department of Energy(DE-FG02-91ER20021).

摘　　要：The Arabidopsis thaliana Calmodulin-binding Transcription Activator(CAMTA)transcription factors CAMTA1,CAMTA2,and CAMTA3(CAMTA123)serve as master regulators of salicylic acid(SA)-mediated immunity,repressing the biosynthesis of SA in healthy plants.Here,we show that CAMTA123 also repress the biosynthesis of pipecolic acid(Pip)in healthy plants.Loss of CAMTA123 function resulted in the induction of AGD2-like defense response protein 1(ALD1),which encodes an enzyme involved in Pip biosynthesis.Induction of ALD1 resulted in the accumulation of high levels of Pip,which brought about increased levels of the SA receptor protein NPR1 without induction of NPR1 expression or requirement for an increase in SA levels.Pip-mediated induction of ALD1 and genes regulating the biosynthesis of SA—CBP60g,SARD1,PAD4,and EDS1—was largely dependent on NPR1.Furthermore,Pip-mediated increase in NPR1 protein levels was associated with priming of Pip and SA biosynthesis genes to induction by low levels of SA.Taken together,our findings expand the role for CAMTA123 in regulating key immunity genes and suggest a working model whereby loss of CAMTA123 repression leads to the induction of plant defense genes and initiation of SAR.

关 键 词：ARABIDOPSIS THALIANA pipecolic ACID salicylic ACID plant IMMUNITY PRIMING CAMTA transcription factors 

分 类 号：Q94[生物学—植物学]