外源性TGF-β1影响肝癌细胞中YAP mRNA及TGF-β1 mRNA的研究  

Effect of ectogenesis TGF-β1 on YAP mRNA level and TGF-β1 mRNA level in hepatocellular carcinoma cells

作　　者：王成 刘承利 张洪义 张辉 赵刚 孔亚林 WANG Cheng;LIU Chengli;ZHANG Hongyi;ZHANG Hui;ZHAO Gang;KONG Yalin(Department of Hepatobiliary Surgery,Air Force Medical Center,Beijing 100142,China;不详)

机构地区：[1]空军特色医学中心肝胆外科,北京100142 [2]首都医科大学附属北京天坛医院普通外科,北京100050

出　　处：《空军医学杂志》2020年第1期45-47,共3页Medical Journal of Air Force

摘　　要：目的研究外源性转化生长因子β1(transforming growth factor-β1,TGF-β1)对97H细胞(人肝癌97细胞系中高转移潜能型)中YAP mRNA及TGF-β1 mRNA水平的影响。方法应用重组人TGF-β1因子作用于97H细胞,再应用荧光定量PCR技术检测YAP mRNA、TGF-β1 mRNA的变化。结果肝癌细胞97H接受外源性TGF-β1因子刺激2 h后出现YAP mRNA的表达下降,此时内源性TGF-β1mRNA无明显变化。结论外源性TGF-β1因子刺激可引起97H细胞YAPmRNA下降,此过程对内源性TGF-β1 mRNA无影响。Objective To study the variation of YAP mRNA and TGF-β1 mRNA in 97 H after being stimulated by ectogenetic TGF-β1 protein.Method Recombinant human TGF-β1 was used to interfere with 97 H growth,and the changes of YAP mRNA and TGF-β1 mRNA were observed by real-time PCR.Results After stimulating 97 H with ectogenetic TGF-β1 for two hours,YAP mRNA decreased,but endogenous TGF-β1 did not change.Conclusion The stimulus of 97 H by ectogenetic TGF-β1 may decrease YAP mRNA levels without affecting endogenous TGF-β1 mRNA levels.

关 键 词：YAP MRNA 转化生长因子Β1 荧光定量PCR 肝细胞癌 

分 类 号：R735.7[医药卫生—肿瘤]