山楂黄酮对BRL-3A肝细胞DNA损伤的修复作用及其机制  

The Protective Effect and Mechanisms of Hawthorn Flavonoids on DNA Damage of BRL-3A Hepatocytes

作　　者：张营霞[1] 王京龙[1] 马晶 卓丽玲[1] 康美玲 田忠景 丁诚实 Zhang Yingxia;Wang Jinglong;Ma Jing;Zhuo Liling;Kang Meiling;Tian Zhongjing;Ding Chengshi(College of Food Science and Pharmaceutical Engineering,Zaozhuang University,Zaozhuang 277160,Shandong;College of Life Science,Zaozhuang University,Zaozhuang 277160,Shandong)

机构地区：[1]枣庄学院食品与制药工程学院,山东枣庄277160 [2]枣庄学院生命科学学院,山东枣庄277160

出　　处：《中国食品学报》2020年第3期90-95,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(81703190);山东省自然科学基金项目(ZR2017LH058);山东省重点研发计划项目(2019GSF107019);山东省高等学校“青创科技计划”(2019KJM006)。

摘　　要：目的:探讨山楂黄酮对BRL-3A肝细胞DNA损伤的影响及其机制。方法:MTT法检测不同浓度的山楂黄酮对乙醇损伤肝细胞的影响;单细胞凝胶电泳分析细胞DNA的损伤程度并计算Olive尾距;试剂盒检测细胞内超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)等生理生化指标;实时定量PCR检测基因SOD的表达变化。结果:0.2,0.4和0.8 mg/mL的山楂黄酮能够显著修复乙醇诱导BRL-3A肝细胞的DNA损伤,这种修复作用与细胞抗氧化指标SOD、MDA和GSH-Px的改善密切相关。结论:山楂黄酮能够通过抗氧化修复肝细胞的DNA损伤,山楂黄酮具备潜在的肝损伤治疗价值。objective:To study the protective effect and mechanisms of hawthorn flavonoids on DNA damage of BRL-3A hepatocytes.Methods:MTT method was used to detect the effect of different concentration hawthorn flavonoids on hepatocytes injured by ethanol.Single-cell gel electrophoresis was used to observe the effect of hawthorn flavonoids on the DNA damage of hepatocytes and the Olive tail moment was measured.Physiological and biochemical indexes such as superoxide dismutase(SOD),malonaldehyde(MDA)and glutathione peroxidase(GSH-Px)in cells were detected by kits.The mRNA expression of the gene SOD was measured by real-time quantitative polymerase chain reaction(qPCR).Results:0.2 mg/mL,0.4 mg/mL and 0.8 mg/mL hawthorn flavonoids could significantly repair the growth and DNA damage of ethanol induced hepatocytes,which was closely related to the improvement of SOD,MDA and GSH-Px.Conclusion:Hawthorn flavonoids can be used to repair liver cells damaged by ethanol,and hawthorn flavonoids have potential for the treatment of liver injury.

关 键 词：山楂黄酮 BRL-3A肝细胞 DNA损伤 生理生化指标 抗氧化 

分 类 号：R285.5[医药卫生—中药学]