痛风雏鹅肾组织损伤的RNA-seq分析  

RNA-seq Analysis on the Renal Tissue Injury in the Development of Goslings Gout

作　　者：邵春荣[1] 黄远丕 黄运茂[2] 应诗家[1] 奚雨萌 施振旦[1] SHAO Chunrong;HUANG Yuanpi;HUANG Yunmao;YING Shijia;XI Yumeng;SHI Zhendan(Animal Husbandry Institute,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Zhongkai University of Agriculture and Engineering,Guangzhou 510000,China)

机构地区：[1]江苏省农业科学院畜牧研究所,南京210014 [2]仲恺农业工程学院,广州510000

出　　处：《畜牧兽医学报》2020年第3期639-643,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31902190);江苏省农业科技自主创新资金项目(CX(18)3024);国家水禽产业技术体系(CARS-42-20)。

摘　　要：试验选取具有典型内脏型痛风特征的雏鹅5只和相同日龄及生长环境下的健康雏鹅6只,利用RNA-seq技术分析其肾组织转录表达差异,旨在了解雏鹅痛风发生的分子机制。结果表明:痛风雏鹅肾组织转录表达显著区别于健康雏鹅。试验共筛选出差异表达基因1 749个,上调基因501个,下调基因1 248个。GO(Gene Ontology)和KEGG(Kyoto Encyclopedia of Genes and Genomes)分析发现,这些差异基因主要具有趋化因子活性、T细胞受体结合、抗原结合等分子功能,参与免疫反应、趋化因子介导信号通路、炎症反应等生物过程,并主要富集于细胞因子受体相互作用、肠道IgA免疫网络、细胞黏附等信号通路。We used RNA-seq to measure the transcription differences in renal tissues between gout and healthy goslings. Five goslings with typical visceral gout characteristics were selected as "gout group", while six healthy goslings were selected as the "control group". As a result, the transcription expressions of gout goslings in renal tissues were significantly different from that of healthy goslings. A total of 1 749 differential expression genes were found. Among these, 501 genes were up-regulated and 1 248 genes were down-regulated in the gout group. The GO and KEGG search revealed the differential expression genes mainly enriched in chemokine activity, T cell receptor binding, antigen binding and immune response, and participate in processes of cytokine-cytokine receptor interaction, intestinal immune network for IgA production, cell adhesion and so on.

关 键 词：雏鹅痛风 肾损伤 RNA-SEQ 免疫应答 炎性损伤 

分 类 号：S856.5[农业科学—临床兽医学]