通心络对脑缺血再灌注大鼠TLR4和NF-κB表达的影响  

Effects of Tongxinluo on expression of TLR4 and NF-κB in rats with cerebralischemia-reperfusion

作　　者：李佳佳[1] 朱云波[1] 康玲伶[1] 朱江[1] LI Jia-Jia;ZHU Yun-Bo;KANG Ling-Ling;ZHU Jiang(Department of Neurology,Affiliated Hospital of Chengde Medical College,Chengde 067000,China)

机构地区：[1]承德医学院附属医院神经内科,承德067000

出　　处：《中国免疫学杂志》2020年第6期672-676,共5页Chinese Journal of Immunology

基　　金：承德市科学技术局科技支撑计划项目(No.201606A050)。

摘　　要：目的:探究通心络对脑缺血再灌注大鼠Toll样受体4(TLR4)和核转录因子(NF-κB)表达的影响。方法:采用线栓法构建脑缺血再灌注(I/R)大鼠模型,将50只SD大鼠随机分为5组,分别为假手术组(Sham)、模型组(I/R)、通心络低剂量组(0.5 g/kg)、通心络高剂量组(2 g/kg)和尼莫地平组(2 mg/kg),每组10只;观察各组大鼠神经功能症状、脑梗死体积和病理组织学变化;采用TUNEL法检测脑组织中细胞凋亡情况,ELISA法检测血清中IL-6、IL-1β和肿瘤坏死因子α(TNF-α)的含量,Western blot法检测脑组织中含半胱氨酸的天冬氨酸蛋白水解酶3(Caspase-3)、TLR4及NF-κB p65的表达。结果:与Sham组相比,I/R组大鼠神经行为学评分、脑梗死体积、脑组织细胞凋亡数、血清IL-6、IL-1β及TNF-α水平、脑组织中Caspase-3、TLR4及NF-κB p65的表达显著升高(P<0.05);给予通心络处理后,大鼠神经行为学评分、脑梗死体积、脑组织细胞凋亡数、血清IL-6、IL-1β及TNF-α水平、脑组织中Caspase-3、TLR4及NF-κB p65的表达显著下降,并呈剂量依赖性(P<0.05)。结论:通心络对脑缺血再灌注大鼠能产生保护作用,减少脑组织细胞的凋亡,可能与下调TLR4、NF-κB的表达有关。Objective:To investigate the effects of Tongxinluo on expression of Toll-like receptor 4(TLR4)and nuclear factor-κB(NF-κB)in rats with cerebral ischemia-reperfusion.Methods:The rat model of cerebral ischemia-reperfusion(I/R)was constructed by suture-occluded method.50 SD rats were randomly divided into 5 groups:Sham operation group(Sham),model group(I/R),Tongxinluo low-dose group(0.5 g/kg),Tongxinluo high-dose group(2 g/kg)and nimodipine group(2 mg/kg),10 cases in each group.The changes in neurological function symptoms,cerebral infarction volume and histopathology in each group were observed.The TUNEL method was performed to detect apoptosis of brain tissues.The contents of serum IL-6,IL-1βand tumor necrosis factorα(TNF-α)were detected by ELISA.Western blot was performed to detect expression of Caspase-3,TLR4 and NF-κB p65 in brain tissues.Results:Compared with those in Sham group,the neurobehavioral score,cerebral infarction volume,number of brain tissue apoptosis,levels of serum IL-6,IL-1βand TNF-α,expression of Caspase-3,TLR4 and NF-κB p65 in brain tissues were significantly increased in I/R group(P<0.05).After treatment with Tongxinluo the above indexes were significantly decreased,showing dose-dependence(P<0.05).Conclusion:There are protective effect of Tongxinluo on rats with cerebral ischemia-reperfusion,which can reduce apoptosis of brain tissues.And that may be related to down-regulation of TLR4 and NF-κB expression.

关 键 词：脑缺血再灌注 通心络 TLR4 NF-ΚB 炎症因子 

分 类 号：R743.31[医药卫生—神经病学与精神病学]