定量检测WT-1基因在急性白血病病人中的表达及临床意义  

Quantitative detection of the WT-1 gene expression in acute leukemia patients and its clinical significance

作　　者：施青青[1] 吴蔚[1] 孙幸[1] 方悦之[1] 朱淼[1] 顾健[1] 倪军[1] SHI Qing-qing;WU Wei;SUN Xing;FANG Yue-zhi;ZHU Miao;GU Jian;NI Jun(Institute of Hematology,Northern Jiangsu People′s Hospital,Yangzhou Jiangsu 225001,China)

机构地区：[1]江苏省苏北人民医院血液病实验室,江苏省扬州市血液学研究所,225001

出　　处：《蚌埠医学院学报》2020年第1期14-17,共4页Journal of Bengbu Medical College

基　　金：江苏省苏北人民医院基金资助项目(yzucms 201102)。

摘　　要：目的:研究Wilms基因(WT-1)在急性髓系白血病(AML)病人骨髓细胞中的表达,并分析其表达与临床疗效的关系。方法:收集AML病人66例,其中急性早幼粒细胞白血病(APL)组8例,急性非淋巴细胞白血病(ANLL,除APL)组58例。对照组20名为健康体检者。采用实时定量PCR法检测骨髓细胞中WT-1表达,并以WT-1的中位表达水平作为界值,将初诊AML病人(APL除外)分为WT-1高表达组和低表达组,并分析其表达与病人性别、年龄、骨髓原幼细胞比例、外周血白细胞数、血红蛋白、血小板数、乳酸脱氢酶(LDH)、C反应蛋白(CRP)、降钙素原(PCT)、完全缓解(CR)率及复发率的关系,以揭示WT-1基因表达水平与AML预后的关系。结果:病例组与对照组相比WT-1表达率差异有统计学意义(χ^2=29.34,P<0.01)。各组WT-1表达水平相比差异有统计学意义(F=7.206,P<0.05),与对照组相比,APL病人和ANLL中M1的病人的WT-1表达水平均较高(P<0.05)。ANLL病人各亚型间相比差异有统计学意义(F=4.981,P<0.05),与M1组病人相比,M2组和M3组病人的WT-1表达水平相对较低(P<0.05);与WT-1低表达组相比,WT-1高表达组中骨髓原幼细胞比例、外周血白细胞数显著升高(P<0.05);而性别、年龄、血红蛋白、血小板数、LDH、CRP、PCT差异无统计学意义(P>0.05),2组病人预后水平差异无统计学意义(P>0.05);AML病人WT-1高表达组CR率低于低表达组(P<0.05)。结论:WT-1在AML病人骨髓细胞中表达水平可作为临床评估疾病的预后、疗效的指标。Objective:To study the expression of Wilms gene(WT-1)in bone marrow cells of patients with acute myeloid leukemia(AML),and analyze the relationship between the expression level of WT-1 and clinical efficacy.Methods:The clinical data of 66 AML patients,which included 8 patients with acute promyelocytic leukemia(APL)and 58 patients with acute non-lymphocytic leukemia(ANLL,except APL),were collected.Twenty healthy people were set as the control group.The expression levels of WT-1 in bone marrow cells of AML patients were detected using real-time quantitative PCR.The median level of WT-1 was set as the clinic value,the 58 newly diagnosed as ANLL patients were divided into the high-expression WT-1 group and low-expression WT-1 group.The relationship between the expression level of WT-1 and sex,age,ratio of progenitor cells,count of peripheral white blood cells,hemoglobin,platelets,lactate dehydrogenase(LDH),C-reactive protein(CRP),calcitonin(PCT),complete remission rate(CR)and relapse rate were analyzed,and the relationship between WT-1 gene expression level and prognosis of AML patients was revealed.Results:The difference of the expression rate of WT-1 between cases group and control group was statistically significant(P<0.01),and the difference of the expression level of WT-1 among each group was statistically significant(P<0.05).Compared with the control group,the expression levels of WT-1 in APL patients and M1 group were higher(P<0.05).The difference of the expression level of WT-1 among ANLL patient subtypes was statistically significant(P<0.05).Compared with the M1 group,the expression levels of WT-1 in M2 and M3 group were relatively low(P<0.05).Compared with the low WT-1 expression group,the proportion of bone marrow progenitor cells and number of peripheral blood white blood cells in high WT-1 expression group significantly increased(P<0.05).There was no statistical significance in gender,age,hemoglobin,platelet count,LDH,CRP,and PCT between low and high WT-1 expression groups(P>0.05),and there was no statis

关 键 词：急性髓系白血病 WT-1基因 实时定量PCR 

分 类 号：R733.71[医药卫生—肿瘤]