非小细胞肺癌中Pokemon蛋白与P14ARF-MDM2-p53通路蛋白的相关性研究  

Study on the correlation between Pokemon protein and P14ARF-MDM2-p53 pathway proteins in non-small cell lung cancer

作　　者：王丽红[1] 窦亚平[1] 韩双双[2] 许浩然[3] 王嘉[1] Wang Lihong;Dou Yaping;Han Shuangshuang;Xu Haoran;Wang Jia(Department of Respiratory Medicine,the First Hospital of Hebei Medical University,Shijiazhuang 050031,China;Department of Endoscope,the First Hospital of Hebei Medical University,Shijiazhuang 050031,China;Department of Respiratory Medicine,Chengde Central Hospital,Chengde 067000,China)

机构地区：[1]河北医科大学第一医院呼吸内科,石家庄050031 [2]河北医科大学第一医院内镜中心,石家庄050031 [3]承德市中心医院呼吸内科,067000

出　　处：《国际呼吸杂志》2020年第4期279-284,共6页International Journal of Respiration

基　　金：河北省科技计划项目(17277735D);河北省财政厅老年病防治项目(LNB201707)。

摘　　要：目的探讨Pokemon蛋白与P14ARF-MDM2-p53通路蛋白在非小细胞肺癌(NSCLC)发病中的作用。方法纳入2015年10月至2016年10月河北医科大学第一医院行手术治疗的35例NSCLC患者为NSCLC组。同时,将癌旁正常支气管断端组织29例及肺炎组织25例分别作为支气管对照组和肺泡对照组。采用免疫组织化学法检测3组中Pokemon、P14ARF、MDM2和p53蛋白的表达情况,分析其与临床病理资料的关系及各蛋白间的相关性。结果Pokemon、MDM2、p53蛋白在NSCLC组中的表达均显著高于支气管对照组和肺泡对照组(P值均<0.01),P14ARF蛋白在NSCLC组中的表达显著低于支气管对照组和肺泡对照组(χ^2=25.39,P<0.01)。p53和MDM2蛋白的表达与NSCLC的分化程度相关(P=0.02、0.03)。在NSCLC组中,Pokemon与P14ARF蛋白表达呈负相关性(r=-0.58,P<0.05),P14ARF与MDM2蛋白表达呈负相关性(r=-0.51,P<0.05),MDM2与p53蛋白的表达呈正相关性(r=0.86,P<0.05)。结论Pokemon通过P14ARF-MDM2-p53通路在NSCLC发病过程中发挥癌基因作用。Objective To investigate the role of Pokemon protein and P14ARF-MDM2-p53 pathway proteins in the pathogenesis of non-small cell lung cancer(NSCLC).Methods This study included 35 NSCLC patients who underwent surgical treatment from the First Hospital of Hebei Medical University from October 2015 to October 2016.At the same time,29 cases of normal bronchus stump adjacent to the cancer and 25 cases of pneumonia were used as the bronchial control group and alveolar control group,respectively.Immunohistochemistry was used to detect the expressions of Pokemon,P14ARF,MDM2 and p53 proteins in NSCLC group,bronchial control group and alveolar control group,and to analyze their relationship with clinicopathological data and their correlations.Results The expressions of Pokemon,MDM2 and p53 proteins in NSCLC group were significantly higher than those in the bronchial control group and the alveolar control group(all P values<0.01).The expression of P14ARF protein in NSCLC group was significantly lower than that in the bronchial control group and alveolar control group(χ^2=25.39,P<0.01).The expressions of p53 and MDM2 protein were correlated with the degree of differentiation of NSCLC(P=0.02,0.03).In NSCLC group,Pokemon was negatively correlated with P14ARF protein expression(r=-0.58,P<0.05),and P14ARF was negatively correlated with MDM2 protein expression(r=-0.51,P<0.05),MDM2 was positively correlated with p53 protein expression(r=0.86,P<0.05).Conclusions Pokemon plays an oncogene role in the pathogenesis of NSCLC by regulating the P14ARF-MDM2-p53 pathway.

关 键 词：癌 非小细胞肺 免疫组织化学 POKEMON P14ARF MDM2 

分 类 号：R734[医药卫生—肿瘤]