中国中小学生微量营养素缺乏风险关联SNP位点民族分布特征  

Ethnic distribution characteristics of SNPs associated with micronutrient deficiency risk of Chinese primary and middle school students

作　　者：张春红 霍军生 孙静 黄建 陈晨 ZHANG Chunhong;HUO Junsheng;SUN Jing;HUANG Jian;CHEN Chen(National Institute for Nutrition and Health,Chinese Center for Disease Control and Prevention,Beijing,100050,China)

机构地区：[1]中国疾病预防控制中心营养与健康所,北京100050 [2]中国人民解放军海军特色医学中心

出　　处：《中国学校卫生》2020年第1期25-28,共4页Chinese Journal of School Health

基　　金：“农村学生营养改善计划”营养健康评估和膳食指导项目资助(2016)。

摘　　要：目的初步探索中国中小学生微量营养素缺乏风险的关联SNP位点地域特征,为评估微量元素缺乏风险提供基础依据。方法搜集以往研究中报道的143个SNP位点,研究对象来自于2016年中国贫困地区营养健康调查项目义务教育中一至九年级1130名中小学生的凝固血细胞,采用磁珠法提取DNA,竞争性等位基因法进行分型检测。对差异基因进行GO显著性富集,采用R软件包进行PCA、kinship和SNP位点之间连锁不平衡分析,分析候选SNP位点的特征。如有种群结构的存在,采用Fa ST-LMM模型进行关联分析。结果差异表达基因在生物过程分组中主要富集在催化活性、转运活性、能量代谢途径、类固醇激素、辅酶、维生素A、维生素D和水溶性维生素的代谢等生物学过程中,涉及转录、翻译以及能量代谢相关基因。143个SNP位点分型结果在民族分布上差异有统计学意义,3号染色体上的基因多态性位点在民族间差异有统计学意义。主成分分析PC1显示,位于RAB6B基因上的rs2280673解释效度在25%以下,位于TF基因上的rs1799852解释效度为25%~50%,位于RBP2基因上的rs2118981位点和SRPRB基因上的rs1830084解释效度在50%~75%之间,位于TF基因上的rs1358024、rs1525892、rs1880669、rs3811647、rs3811658、rs6794945、rs7638018、rs8177248等8个位点的解释效度为75%以上。结论中国中小学生微量营养素缺乏风险关联SNP位点存在民族分布差异。Objective To explore ethnic distribution characteristics of SNPs associated with micronutrient deficiency risk of Chinese primary and middle school students,and to provide a basic reference for evaluating the risk of lack in micronutrient.Methods Totally 143 SNPs reported in previous studies were collected,and DNA was exacted by using magnetic beads in frozen blood cell samples from the 2016 nutrition health survey project of 1130 primary and middle school students,competitive allele method was used to detect SNP genotyping.GO significant enrichment analysis R software package to PCA,kinship and linkage disequilibrium analysis were used for analysis of features of candidate SNPs.If there was a population structure,the Fa ST-LMM model was used for correlation analysis.Results The GO significant enrichment results showed that differentially expressed genes were mainly enriched in the biological process grouping,including catalytic activity,transport activity,energy metabolism pathway,steroid hormone,coenzyme,biological processes of vitamin A,D and metabolism of water-soluble vitamins,involving transcription,translation and energy metabolism related genes.The results of 143 SNPs showed statistically significant differences in ethnic distribution,and SNPs on chromosome 3 presented significant differences among ethnic groups.Principal component analysis 1 showed that rs1799852 on TF gene had 25%-50%explanatory validity,rs2118981 on RBP2 gene and rs1830084 on SRPRB gene had 50%-75%explanatory validity,rs1358024,rs1525892,rs1880669,rs3811647,rs3811658,rs6794945,rs7638018 and rs8177248 on TF gene had more than 75%explanatory validity.Conclusion SNPs associated with micronutrient deficiency risk of Chinese primary and middle school students are characterized with ethnic distributions.

关 键 词：微量营养系 儿童营养障碍 多态性 单核苷酸 基因 血液化学分析 学生 

分 类 号：R151[医药卫生—营养与食品卫生学] R446[医药卫生—公共卫生与预防医学]