联合转染PTEN与PINCH siRNA对结直肠癌细胞增殖、侵袭及凋亡影响的机制研究  

The effect and mechanism of combined transfection of PTEN and PINCH siRNA on proliferation,invasion and apoptosis of colorectal cancer cells

作　　者：巴云涛 王权 刘海龙 李志猛 常玉玺 孙浩 Ba Yuntao;Wang Quan;Liu Hailong;Li Zhimeng;Chang Yuxi;Sun Hao(Department of Radiotherapy,Tumor Hospital Affiliated to Zhengzhou University,Henan Zhengzhou 450008,China)

机构地区：[1]郑州大学附属肿瘤医院放疗科,河南郑州450008

出　　处：《现代肿瘤医学》2020年第8期1252-1257,共6页Journal of Modern Oncology

基　　金：河南省科技攻关项目(编号:162102310323,162102310328)。

摘　　要：目的:研究联合转染PTEN与PINCH siRNA对结直肠癌SW620细胞增殖、侵袭和凋亡的影响。方法:在结直肠癌SW620细胞中转染pcDNA-PTEN或/和si-PINCH,qRT-PCR检测PINCH和PTEN mRNA的表达,Western blot检测PINCH、PTEN、CDK1、MMP-2、Bcl-2和Bax蛋白表达,MTT法、Transwell实验和流式细胞术分别检测细胞增殖、侵袭能力和凋亡率。结果:在结直肠癌SW620细胞中联合转染pcDNA-PTEN和si-PINCH后,PTEN的mRNA和蛋白表达量显著升高(P<0.05),PINCH的mRNA和蛋白表达量显著降低(P<0.05);转染pcDNA-PTEN或/和si-PINCH均可抑制SW620细胞增殖和侵袭并促进细胞凋亡,抑制SW620细胞内增殖蛋白CDK1、侵袭蛋白MMP-2和抗凋亡蛋白Bcl-2的表达,促进凋亡蛋白Bax的表达;联合转染pcDNA-PTEN和si-PINCH比单独转染pcDNA-PTEN或si-PINCH效果更显著。结论:联合转染pcDNA-PTEN和si-PINCH可抑制结直肠癌SW620细胞增殖和侵袭并促进细胞凋亡,且比单独转染效果更显著。Objective:To investigate the effect of combined transfection of PTEN and PINCH siRNA on proliferation,invasion and apoptosis of colorectal cancer SW620 cells.Methods:pcDNA-PTEN or/and si-PINCH were transfected into colorectal cancer SW620 cells.The mRNA expression levels of PINCH and PTEN were detected by qRT-PCR.The protein expression levels of PINCH,PTEN,CDK1,MMP-2,Bcl-2 and Bax were determined by Western blot.The proliferation,invasion and apoptotic rate were measured by MTT,Transwell and flow cytometry,respectively.Results:After co-transfection of pcDNA-PTEN and si-PINCH into SW620 cells,the expression levels of PTEN mRNA and protein were increased significantly(P<0.05),and that expression levels of PINCH were decreased remarkably(P<0.05).Transfection of pcDNA-PTEN or/and si-PINCH inhibited the proliferation and invasion of SW620 cells and promoted cell apoptosis,inhibited the expression of proliferation-related protein CDK1,invasion-related protein MMP-2 and anti-apoptotic protein Bcl-2 in SW620 cells,and promoted the expression of apoptotic protein Bax.Co-transfection of pcDNA-PTEN and si-PINCH was more effective than transfection of pcDNA-PTEN or si-PINCH alone.Conclusion:Co-transfection of pcDNA-PTEN and si-PINCH inhibited the proliferation and invasion of colorectal cancer SW620 cells and promoted apoptosis,which was more significant than transfection alone.

关 键 词：结直肠癌 PTEN PINCH 增殖 侵袭 凋亡 

分 类 号：R735.3[医药卫生—肿瘤]