盐酸罗哌卡因对胃癌MGC-803细胞增殖和凋亡的作用  

Effect of ropivacaine on proliferation and apoptosis of gastric cancer MGC-803 cells

作　　者：朱婧 张世平[1] 张文娟 李鑫 宋宇龙[1] Zhu Jing;Zhang Shiping;Zhang Wenjuan;Li Xin;Song Yulong(Department of Anaesthesiology,Shaanxi Provincial People's Hospital,Shaanxi Xi'an 710068,China)

机构地区：[1]陕西省人民医院麻醉科,陕西西安710068

出　　处：《现代肿瘤医学》2020年第8期1261-1265,共5页Journal of Modern Oncology

基　　金：陕西省自然科学基础研究计划项目(编号:2018JM7121)。

摘　　要：目的:探讨盐酸罗哌卡因对人胃癌MGC-803细胞增殖和凋亡的影响。方法:细胞计数试剂盒(CCK-8)检测盐酸罗哌卡因对MGC-803细胞增殖能力的影响,并确定盐酸罗哌卡因的用药浓度,流式细胞术检测盐酸罗哌卡因对MGC-803细胞周期的影响,Annexin V-FITC/PI法检测盐酸罗哌卡因对MGC-803细胞凋亡的影响,蛋白免疫印迹法(Western blot)检测盐酸罗哌卡因对MGC-803细胞中B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)和剪切的含半胱氨酸的天冬氨酸蛋白水解酶3(Cleaved Caspase-3)蛋白表达水平。结果:随着盐酸罗哌卡因用药浓度的升高,MGC-803细胞增殖能力逐渐降低,根据CCK-8实验结果分别筛选出浓度为10、50μg/ml和100μg/ml的盐酸罗哌卡因用于后续实验。盐酸罗哌卡因能够明显阻滞细胞周期于G2期,诱导细胞凋亡,抑制Bcl-2蛋白表达,促进Bax和Cleaved Caspase-3蛋白表达。结论:盐酸罗哌卡因能够抑制胃癌MGC-803细胞增殖,阻碍MGC-803细胞周期进程,诱导细胞凋亡,该过程可能与下调Bcl-2蛋白表达,上调Bax和Cleaved Caspase-3蛋白表达有关。Objective:To investigate the effect of ropivacaine on proliferation and apoptosis of human gastric cancer MGC-803 cells.Methods:Cell counting kit(CCK-8)was used to detect the effect of ropivacaine on the proliferation of MGC-803 cells,and the concentration of ropivacaine was determined.Flow cytometry was used to detect ropivacaine on the cell cycle of MGC-803.The effect of tranexaine on apoptosis of MGC-803 cells was detected by Annexin V-FITC/PI method.Western blot was used to detect Bcl-2,Bax and Cleaved Caspase-3 protein expression levels.Results:With the increase of ropivacaine concentration,the proliferation of MGC-803 cells gradually decreased.According to the results of CCK-8,the concentrations of ropivacaine at 10,50μg/ml and 100μg/ml were screened for subsequent experiments.Ropivacaine can significantly block the cell cycle in G2 phase,induce apoptosis,inhibit Bcl-2 protein expression,and promote the expression of Bax and Cleaved Caspase-3 protein.Conclusion:Ropivacaine can inhibit the proliferation of gastric cancer MGC-803 cells,block the cell cycle progression of MGC-803,and induce apoptosis.This process may be related to down-regulating Bcl-2 protein expression and up-regulating Bax and Cleaved Caspase-3 protein expression.

关 键 词：盐酸罗哌卡因 胃癌MGC-803细胞 细胞增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]