高效液相色谱法测定曲札茋苷原料药的含量及有关物质  

Content determination of piceatannol-3’-O-β-D-glucopyranoside and related substances by HPLC

作　　者：孙玉明[1] 柴洪杰 张华[1] 王静[1] 王玉林[2] SUN Yu-ming;CHAI Hong-jie;ZHANG Hua;WANG Jing;WANG Yu-lin(Dalian University of Technology,School of Chemical Engineering,Dalian Liaoning 116024;Dalian Medical University,Basic Medical College,Dalian Liaoning 116044)

机构地区：[1]大连理工大学化工学院,辽宁大连116024 [2]大连医科大学基础医学院,辽宁大连116044

出　　处：《中南药学》2020年第2期246-249,共4页Central South Pharmacy

摘　　要：目的建立高效液相色谱法测定曲札茋苷的含量及有关物质。方法色谱柱:Agilent Zorbax SBC18柱(150 mm×4.6 mm,5μm);流动相:乙腈-水溶液;流速:1.0 mL·min^-1;柱温:30℃;进样量:20μL;检测波长:319 nm。结果结果表明曲札茋苷在2~20μg·mL^-1与峰面积呈现良好的线性相关性(r=0.9999);定量限和检测限分别为0.80和0.26 ng;回收率在98.0%~102.0%;各杂质与主峰的分离度良好。结论该方法操作简单,准确度高,精密度好,可用于曲札茋苷的含量及有关物质检测。Objective To establish an HPLC-UV method to determine the content and related substances of piceatannol-3’-O-β-D-glucopyranoside.Methods The separation was performed on Agilent Zorbax SB-C18 column(150 mm×4.6 mm,5μm).The detection wavelength was 319 nm and the mobile phase was acetonitrile and water at a flow rate of 1.0 mL·min^-1.The column temperature was 30℃,and the injection volume was 20μL.Results Piceatannol-3’-O-β-D-glucopyranoside showed a good linearity at 2-20μg·mL^-1(r=0.9999).The limit of quantification and detection was 0.80 and 0.26 ng.The accuracy was at 98.0%-102.0%.The related substances were all detected with good resolution.Conclusion The method is simple,accurate and precise,which can be used to the determine the content and related substances of piceatannol-3’-O-β-D-glucopyranoside.

关 键 词：高效液相色谱法 曲札茋苷 含量测定 有关物质检测 

分 类 号：R917[医药卫生—药物分析学] R927[医药卫生—药学]