乙型肝炎病毒x蛋白抗体及细胞因子在乙型肝炎和肝癌患者中的意义  

The clinical significance of anti-HBx and cytokines in patients with chronic hepatitis B and liver cancer

作　　者：段正军[1] 李彩东[1] 杨勇卫[1] 田鹏飞[1] 陈锡莲[1] DUAN Zheng-jun;LI Cai-dong;YANG Yong-wei;TIAN Peng-fei;CHEN Xi-lian(Liver Diseases Research Institute,the Second People's Hospital of Lanzhou,Lanzhou,Gansu 730046,China)

机构地区：[1]兰州市第二人民医院肝病研究所,甘肃兰州730046

出　　处：《中国卫生检验杂志》2020年第2期186-188,191,共4页Chinese Journal of Health Laboratory Technology

基　　金：兰州市人才创新创业项目(2015-RC-66)。

摘　　要：目的探讨乙型肝炎患者外周血中抗-HBx蛋白和细胞因子TNF-α、IL-4、IL-12表达水平。方法选择HBV携带者60例(ASC组),慢性乙型肝炎患者60例(CHB组),肝硬化患者60例(LC组),肝癌患者60例(HCC组),健康体检者60例(对照组)为研究对象,采用酶联免疫法检测抗-HBx、TNF-α、IL-4、IL-12表达水平,实时荧光定量PCR法检测HBV DNA载量。结果与对照组比较,ASC组、CHB组、LC组和HCC组外周血TNF-α、IL-4和IL-12水平均显著升高(P<0.01);与HCC组相比,ASC组和CHB组外周血抗-HBx水平和IL-12水平均显著升高,差异均有统计学意义(P<0.05)。HCC组外周血抗-HBx、TNF-α和IL-4水平与HBV DNA载量均呈负相关(r值分别为-0.408、-0.348、-0.373,P<0.05)。结论抗-HBx蛋白在原发性肝癌发生、发展过程中有一种独特作用机制,与HCC组相比,ASC组和CHB组外周血抗-HBx水平和IL-12水平均显著升高,说明慢性HBV感染者在疾病发展过程中均存在不同程度的免疫炎症反应。Objective To investigate the value and clinical significance of anti-HBx and cytokines(TNF-α,IL-4,IL-12)in patients with HBV.Methods A total of 60 cases of HCC,60 cases of LC,60 cases of CHB,60 cases of ASC,60 cases of non-HBV infected individuals(controls)were selected to detect the levels of anti-HBx,TNF-α,IL-4,IL-12 by enzyme-linked immunoadsordent assay(ELISA).The HBV DNA was measured by real-time quantitative polymerase chain reaction.Results Compared with those in health controls,cytokines levels in HCC,LC,CHB,ASC groups significant increased(P<0.01).Compared with the HCC group,anti-HBx level significant increased in ASC and CHB groups(P<0.05).Anti-HBx,TNF-α and IL-4 levels were negatively correlated to HBV DNA in HCC group(r=-0.408,r=-0.348,r=-0.373;P<0.05).Conclusion Anti-HBx protein has a unique mechanism of action in the occurrence and development of primary liver cancer.Compared with the HCC group,the peripheral blood anti-HBx levels and IL-12 levels in the ASC and CHB groups significantly increased,indicating that the patients with chronic HBV have different degrees of immune inflammation during the development of the disease.

关 键 词：慢性乙型肝炎 乙型肝炎病毒x蛋白抗体 白细胞介素-4 白细胞介素-12 乙型肝炎病毒DNA 

分 类 号：R735.7[医药卫生—肿瘤]