重庆工业园区土壤微生物群落结构及功能分析--以焦化厂土壤为例  

Evaluation of Microbial Community Structure and Functional Characteristics in Industrial Park Soil--a Case Study of Coking Plant Soil

作　　者：邢志林[1] 袁山林 郭江枫 张正义 肖芃颖[1] 姚源[2] 曹昆 XING Zhilin;YUAN Shanlin;Guo Jiangfeng;ZHANG Zhengyi;XIAO Pengying;YAO Yuan;CAO Kun(Colleae of Chemistry and Chemical Engineering,Chongqing Universite of Technolovy,Chongqing 400050,China;Chongqing Academy of Ecclooical and Environmental Sciences,Chongqing 401147,China)

机构地区：[1]重庆理工大学化学化工学院,重庆400050 [2]重庆市环境科学研究院,重庆401147

出　　处：《环境影响评价》2020年第2期74-78,共5页Environmental Impact Assessment

基　　金：重庆市生态环境局科研项目资助(2019-21);重庆理工大学科研立项项目资助(KLA18015)。

摘　　要：研究了重庆工业园区焦化厂污染土壤的微生物群落结构及功能特性,检测了污染土壤中的多环芳烃和氯代芳烃种类和浓度,检测出11种多环芳烃和3种典型氯代芳烃,多环芳烃浓度范围为921~37 390 ng/g,氯代芳烃含量范围9 542~12 301 ng/g,其中1,4-二氯苯浓度最高;多样性测序结果表明污染土壤中微生物丰富度和多样性减少,实现了功能菌属的富集,随着污染物浓度增大,独有微生物数量呈倍数下降,污染浓度最高的样品中独有的微生物的重要菌属为Rhodanobacter;功能分析结果表明Pseudomonas,Rhodanobacter,Hydrogenophaga,Dyella和Xanthomonadaceae5种功能微生物具有降解多种多环芳烃和氯代芳烃的能力;表明污染土壤具有强的原位生物修复能力。The types and concentrations of PAHs and chlorinated aromatics in the contaminated soil were detected,and 11 PAHs and 3 typical chlorinated aromatics were detected,ranging from 921 ~ 37 390 ng/g and 9 542 ~ 12 301 ng/g,among which the concentration of1,4-dichlorobenzene was the highest,the diversity sequencing results show that the microbial richness and diversity in contaminated soil decrease,and the enrichment of functional bacteria is realized. And then with the increase of pollutant concentration,the number of unique microorganisms decreases by multiple. The important genus of unique microorganisms in the samples with the highest pollution concentration is Rhodanobacter. Functional analysis results showed that five functional microorganisms Pseudomonas,Rhodanobacter,Hydrogenophaga,Dyella and Xanthomonadaceae have the ability to degrade a variety of polycyclic aromatic hydrocarbons and chlorinated aromatic hydrocarbons. All shows that contaminated soil has strong in situ bioremediation ability.

关 键 词：焦化厂 多环芳烃 氯代芳烃 微生物多样性 微生物降解 

分 类 号：X53[环境科学与工程—环境工程]