miR-34和IMP3在卵巢癌组织中的表达及临床意义  

Expressions and clinical significances of MiR-34 and IMP3 in ovarian cancer

作　　者：叶芳丽[1] 将翡翎 潘在兴[1] 魏小斌[1] 吴翔宇[1] YE Fangli;JIANG Feiling;PAN Zaixing;WEI Xiaobin;WU Xiangyu(Department of Clinical Laboratory,Haikou People′s Hospital,Haikou,Hainan 570208,China)

机构地区：[1]海口市人民医院检验科,海南海口570208

出　　处：《国际检验医学杂志》2020年第7期856-860,866,共6页International Journal of Laboratory Medicine

摘　　要：目的探讨微小RNA-34(miR-34)和胰岛素样生长因子ⅡmRNA结合蛋白3(IMP3)在卵巢癌组织中的表达关系及临床意义。方法收集2016年2月至2018年12月该院就诊卵巢癌患者手术切除后的癌组织标本92例为卵巢癌组,另取相应癌旁组织为对照组。采用实时荧光定量(qPCR)法检测miR-34与IMP3 mRNA相对表达量,采用免疫组化法检测IMP3蛋白表达。根据检测结果将miR-34分为高表达组(42例)、低表达组(50例),IMP3阳性表达组(54例)、阴性表达组(38例),观察其与卵巢癌患者临床病理参数的关系。采用Pearson法分析卵巢癌患者miR-34与IMP3相关性;采用Kaplan-Meier分析卵巢癌患者3年生存情况。结果卵巢癌组miR-34表达水平显著低于对照组,而IMP3 mRNA及蛋白表达均显著高于对照组,差异有统计学意义(P<0.05);miR-34、IMP3均与病理分级、淋巴结转移、临床分期、分化程度及腹腔积液转移有关(χ21=13.401、9.405、15.756、10.683、4.843;χ22=27.819、18.386、15.222、7.188、4.392,P<0.05);miR-34与IMP3呈显著负相关(r=-0.608,P=0.000);Kaplan-Meier分析显示miR-34高表达组患者PFS与OS均显著高于低表达组,IMP3阴性表达组患者PFS与OS均显著高于阳性表达组,差异有统计学意义(P<0.05)。结论miR-34在卵巢癌中表达水平降低,而IMP3表达水平升高,两者均可为卵巢癌诊断提供依据并可作为监测卵巢癌患者预后的重要生物学指标。Objective To investigate the expression of micro RNA-34(miR-34)and insulin-like growth factor II mRNA binding protein 3(IMP3)in ovarian cancer and its clinical significance.Methods From February 2016 to December 2018,92 cases of ovarian cancer tissue specimens surgically resected from the patients were collected as ovarian cancer group,and the corresponding para cancerous tissues were taken as normal group.The relative expressions of miR-34 and IMP3 mRNA were detected by real-time fluorescence quantitative(qRT-PCR),immunohistochemistry was used to detect the expression of IMP3 protein.According to the test results,the patients were divided into miR-34 high expression group(42 cases)and low expression group(50 cases),IMP3 positive expression group(54 cases)and negative expression group(38 cases),observed their relationships with clinicopathological parameters of ovarian cancer patients.Pearson method was used to analyze the correlation between miR-34 and IMP3 in patients with ovarian cancer;Kaplan-Meier analysis was used to analyze the 3-year survival of patients with ovarian cancer.Results The expression level of miR-34 in ovarian cancer group was significantly lower than that in normal group,the expressions of IMP3 mRNA and protein were significantly higher than those in the normal group,the differences were statistically significant(P<0.05);MiR-34 and IMP3 were related to pathological grade,lymph node metastasis,clinical stage,differentiation degree and ascites metastasis(χ21=13.401,9.405,15.756,10.683,4.843;χ22=27.819,18.386,15.222,7.188,4.392,P<0.05);there was a significant negative correlation between miR-34 and IMP3(r=-0.608,P=0.000);Kaplan-Meier analysis showed that PFS and OS in miR-34 high expression group were significantly higher than those in low expression group,PFS and OS in IMP3 negative expression group were significantly higher than those in positive expression group,the differences were statistically significant(P<0.05).Conclusion The expression level of miR-34 in ovarian cancer is decreased,t

关 键 词：微小RNA-34 胰岛素样生长因子ⅡmRNA结合蛋白3 卵巢癌 

分 类 号：R737.31[医药卫生—肿瘤]