异养菌与新型填料成膜性及BTF处理屠宰H2S废气  

Biofilm formation of a heterotrophic bacterium on the novel packing material and the removal of hydrogen sulfide produced in a slaughtering process by biotrickling filter

作　　者：庄荣玉[1] 王磊刚 李美燕 任雨婷 潜丽珍 高华生[6] 刘军 赵洋甬 陈健 朱红青 ZHUANG Rong-yu;WANG Lei-gang;LI Mei-yan;REN Yu-ting;QIAN Li-zhen;GAO Hua-sheng;LIU Jun;ZHAO Yang-yong;CHEN Jian;ZHU Hong-qing(Key Laboratory of Animal Protein Foods Deep Processing of Zhejiang Province,College of Food and Pharmaceutical Sciences,Ningbo University,Ningbo 315800,China;Ningbo Qingyun Environmental Protection Technology Co.,Ltd.,Ningbo 315034,China;Yiwu Zhongde After-School Education Guidance Station Co.,Ltd.,Yiwu 322000,China;Zhejiang Jianchuang Testing and Technology Service CO.,Ltd.,Hangzhou 310051,China;Zhejiang Xinkongshun Food Co.,Ltd.,Lishui 321400,China;School of Civil and Environmental Engineering,Ningbo University,Ningbo 315211,China;Guangdong Guoqian Environmental and Energy-Saving Technology Institute,Co.,Ltd.,Guangzhou 510663,China;Zhejiang Easy Testing Environmental Technology Co.,Ltd.,Ningbo 315100,China)

机构地区：[1]宁波大学食品与药学学院,浙江省动物蛋白食品精深加工重点实验室,浙江宁波315800 [2]宁波青云环保科技有限公司,浙江宁波315034 [3]义乌市重德校外教育辅导站有限公司,浙江义乌322000 [4]浙江检创检测技术服务公司,浙江杭州310051 [5]浙江鑫康顺食品有限公司,浙江丽水321400 [6]宁波大学土木与环境工程学院,浙江宁波315211 [7]广东国千环境与节能技术研究院有限公司,广东广州510663 [8]浙江易测环境科技有限公司,浙江宁波315100

出　　处：《中国环境科学》2020年第3期1106-1115,共10页China Environmental Science

基　　金：浙江省科技厅公益技术应用研究计划项目(2017C33009);宁波市科技局科技富民项目(2017C10034);宁波市科技局科研计划项目(2016C51034)。

摘　　要：以新型填料上生物膜的微生物种群结构为研究对象,将异养脱硫细菌蜡状芽孢杆菌ZJNB-B3接种到含活性污泥和LEVAPOR新型填料的小试曝气液体反应器,研究此菌在活性污泥及在填料生物膜中的微生物多样性.在属分类水平上的物种分析结果表明,芽孢杆菌属在接种后的填料中相对丰度较高,而在活性污泥样品中相对丰度较低,其在培养了第10d的填料样品中已经达到最大相对丰度.因此该菌与新型填料的亲和性和成膜性较好.将该菌与新型填料应用于生物滴滤塔(BTF)处理屠宰污水站排放的H2S恶臭废气,风量处理能力为2000m^3/h.结果表明,经该菌强化的BTF完成启动的时间比使用普通活性污泥接种的BTF缩短7d.强化的BTF在30d的稳定期试验中,当H2S进气浓度为4.92-9.54mg/m^3时,对H2S去除率为98%-99%.当进气H 2S在1.0-10mg/m^3范围波动时,空床停留时间(EBRT)为10,21,30s时,去除率分别为94%、98%、99%以上,且受进气H2S浓度波动的影响不大.当进口负荷为0.60-1.14g/(m^3·h)时,H2S去除率稳定在98%以上,H2S气体排放量低于恶臭污染物排放国家标准.The microbial taxonomic composition distribution of biofilm on the novel packing material was studied.The heterotrophic desulfurization bacterium Bacillus cereus ZJNB-B3 was inoculated and injected into an aeration liquid reactor containing activated sludge and LEVAPOR novel packing material.The microbial diversity of the bacteria in the reactor was evaluated.The taxonomic composition distribution of Genus-level showed that Bacillus had a higher relative abundance on the LEVAPOR packing material while lower in activated sludge.On the 10th day of the aeration reaction,the maximum relative abundance of the Bacillus on the LEVAPOR packing samples was achieved.Therefore,the Bacillus cereus ZJNB-B3 strain has excellent affinity with LEVAPOR packing material.Afterward,the removal of hydrogen sulfide of slaughtering sewage by biotrickling filter(BTF)inoculated with the desulfurization bacterium was investigated.The treatment capacity was 2000m^3/h.The start-up period of the BTF inoculated with the desulfurization bacterium was 7days,which was 7days shorter than that of the BTF only inoculated with activated sludge.The stable operation of the enhanced BTF was carried out for 30days.When the inlet concentration of H2S was 4.92-9.54mg/m^3,the removal efficiency(RE)for H2S was achieved by 98%-99%.When the empty bed residence time(EBRT)were 10s,21s,and 30s,the RE was above 94%,98%and 99%respectively.And the inlet H2S concentration of 1.0-10mg/m^3 had no obvious influence on the RE.When the inlet load was 0.60-1.14g/(m^3·h),the RE was above 98%.The emission of H2S was lower than the national emission standards for odor pollutants.

关 键 词：异养菌 LEVAPOR新型填料 生物膜 微生物多样性 生物滴滤塔 屠宰加工厂污水站 硫化氢 

分 类 号：X701[环境科学与工程—环境工程]