苦丁茶不同提取物抗氧化活性比较及其GC-MS分析  

Antioxidant Activities and GC-MS Analysis of Ilex kudingcha C.J. Tseng Extracts by Different Extraction Methods

作　　者：朱科学[1,2] 刀春丽 顾文亮 徐飞[1] 谭乐和[1,2] ZHU Kexue;DAO Chunli;GU Wenliang;XU Fei;TAN Lehe(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning,Hainan 571533,China;Hainan Provincial Engineering Research Center for Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China;College of Tropical Crop Science,Yunnan Agricultural University,Pu’er,Yunnan 665000,China)

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]海南省热带香料饮料作物工程技术研究中心,海南万宁571533 [3]云南农业大学热带作物学院,云南普洱665000

出　　处：《热带作物学报》2020年第3期579-585,共7页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院基本科研业务费专项资金(No.1630142017019)。

摘　　要：利用气相色谱-质谱联用技术(GC-MS),对苦丁茶超声辅助不同有机溶剂(乙醇、乙酸乙酯、石油醚)提取物及超临界CO2萃取物的体外抗氧化活性(DPPH自由基清除能力、羟基自由基清除活性、超氧阴离子自由基清除活性和ABTS+自由基清除能力)进行比较分析。结果表明,苦丁茶不同提取物DPPH清除能力和超氧阴离子自由基清除活性依次为:超声-乙醇提取物>超临界CO2萃取物>超声-乙酸乙酯提取物>超声-石油醚提取物,羟基自由基和ABTS+自由基清除能力依次为:超声-乙醇提取物>超声-乙酸乙酯提取物>超临界CO2萃取物>超声-石油醚提取物。GC-MS结果显示,苦丁茶不同提取物中主要有5类物质,共有49种化学成分,7种共有成分。The present study was aimed to compare the effects of the extracts of Ilex kudingcha C.J. Tseng with different organic solvents(ethanol, ethyl acetate, petroleum ether) assisted by ultrasound and supercritical CO2 extraction against oxidation activities(DPPH·) scavenging activity, hydroxyl radical(·OH) elimination activity, superoxide anion free radical(O2-·) scavenging activity and ABTS+· radical elimination ability), and component analysis by gas chromatography-mass spectrometry(GC-MS). The DPPH· and O2-· scavenging abilities were as follows: ultrasound-ethanol extract > supercritical CO2 extract > ultrasound-ethyl acetate > ultrasound-petroleum ether extract. The OH· and ABTS+· radical scavenging abilities were as follows: ultrasound-ethanol extract > ultrasound-ethyl acetate extract > supercritical CO2 extract > ultrasound-petroleum ether extract. GC-MS analysis showed that there were five main types of substances in the four different extraction methods, a total of 50 chemical components and seven common components.

关 键 词：苦丁茶 提取物 超临界CO2 体外抗氧化活性 气相色谱-质谱 

分 类 号：R284.1[医药卫生—中药学] Q946.91[医药卫生—中医学]