先天性双侧输精管缺如患者睾丸组织中CFTR蛋白的表达及意义  

The expression and significance of CFTR protein of testis in Chinese congenital bilateral absence of the vas deferens

作　　者：白松[1] 吴斌[1] Bai Song;Wu Bin(Urology Department of Shengjing Hospital Affiliated China Medical University,Shenyang,China 110004)

机构地区：[1]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004

出　　处：《中国男科学杂志》2020年第1期24-27,共4页Chinese Journal of Andrology

摘　　要：目的通过观察中国CBAVD患者CFTR蛋白在睾丸组织中的表达水平,探讨其与睾丸生精功能之间的关系。方法免疫组化检测66例中国先天性双侧输精管缺如患者睾丸组织CFTR蛋白表达水平并对睾丸生精功能进行评分,探讨先天性双侧输精管缺如患者CFTR蛋白的表达情况以及其与睾丸生精功能的关系。结果免疫组化结果显示CFTR蛋白可表达于睾丸内支持细胞及生精上皮细胞,以细胞膜和细胞浆为主;CFTR蛋白在CBAVD患者睾丸生精上皮及支持细胞的表达以阳性和弱阳性为主,比例分别为50%(33/66)和37.87%(25/66),部分患者可见阴性表达,比例为4.54%(3/66);睾丸生精Johnsen评分7~10分的比例分别为4.45%(3/66)、40.9%(27/66)、42.42%(28/66)和12.23%(8/66)。部分患者存在生精功能障碍。CFTR蛋白在睾丸组织中的表达与生精功能强正相关性,相关系数为0.785(P<0.01)。结论BAVD患者睾丸内的CFTR蛋白表达以阳性和弱阳性为主;部分CBAVD患者存在生精功能障碍,CFTR蛋白在睾丸组织中的表达水平与生精功能存在强正相关性。Objective To observe the CFTR protein expression level and its association with spermatogenesis in Chinese CBAVD.Methods Immunohistochemical method detect CFTR protein expression level and evaluate spermatogenesis function in 66 cases of CBAVD.To evaluate the expression level of CFTR protein and spermatogenesis function.Results Immunohistochemical results reveal CFTR protein expressed in sertoli cell and spermatogenic epithelium cell of testis,mainly in cytoplasm and cytomembrane.Expression level of CFTR protein in CBAVD is mainly positive and weak positive which are 50%(33/66)and 37.87%(25/66),4.54%(3/66)are negative.Johnsen score of CBAVD group are from 7 to 10 and the proportion is 4.45%,40.9%,42.42%and 12.23%respectively according to score.The correlation coefficient is 0.785 between spermatogenesis function and CFTR protein expression level.Conclusions Part of them has lower expression level in CBAVD and lower spermatogensis function also.CFTR protein expression level has strongly positive correlation with spermatogensis function.

关 键 词：CFTR蛋白 先天性双侧输精管缺如 生精功能 睾丸 

分 类 号：R697.2[医药卫生—泌尿科学] R341.3[医药卫生—外科学]