一种用于microRNA-21浓度检测的荧光传感方法的建立  

Establishment of a fluorescence sensing method for microRNA-21concentration detection

作　　者：章德昆 陈婷婷 陈魁 王力群 龚超 李嵩 夏垚坤 陈敬华[2] ZHANG Dekun;CHEN Tingting;CHEN Kui;WANG Liqun;GONG Chao;LI Song;XIA Yaokun;CHEN Jinghua(The Affiliated Fuzhou Municipal First Hospital of Fujian Medical University,Fuzhou,Fujian 350009,China)

机构地区：[1]福建医科大学附属福州市第一医院,福州350009 [2]福建医科大学药学院

出　　处：《福建医药杂志》2020年第1期132-134,F0003,共4页Fujian Medical Journal

基　　金：福建省自然科学基金(2017J01226)。

摘　　要：目的建立一种用于microRNA-21(miRNA-21)浓度检测的,基于聚多巴胺纳米粒子(PDANs)的荧光传感方法。方法Ce6标记的捕获探针(Ce6-DNA)在Ca^2+的介导下吸附在PDANs表面,荧光被猝灭。miRNA-21和Ce6-DNA杂交后,使其从PDANs表面解吸附,荧光恢复。利用荧光分光光度计分析靶标和Ce6-DNA反应前后荧光强度的变化,实现对miRNA-21的检测。结果在5.0~200nM范围内,670nm处的荧光强度和miRNA-21的浓度呈良好的线性关系,检测限为1.80nM。结论基于Ce6-DNA功能化的PDANs可为miRNA-21的定量检测提供一种简单、准确、成本低、分析速度快的新方法。Objective To develop a fluorescent method based on polydopamine nanoparticles(PDANs)for microRNA-21(miRNA-21)concentration detection.Methods Ce6-labeled capture probe(Ce6-DNA)was absorbed on the surface of PDANs through Ca^2+mediation,which resulted in the fluorescence of Ce6being quenched.However,the Ce6-DNA was desorbed from PDANs when it hybridized with miRNA-21,which led to the recovery of fluorescence.This method could detect miRNA-21 quantitatively through measuring the change of fluorescence intensity using fluorescent spectrophotometer.Results There was a good linear relationship between the fluorescence intensity at 670nm and miRNA-21concentration from 5.0to 200nM.The limit of detection was 1.80nM.Conclusion The Ce6-DNA functionalized PDANs can provide a simple,accurate,low-cost,and rapid novel method for miRNA-21detection.

关 键 词：聚多巴胺纳米粒子 MIRNA-21 DNA探针 荧光检测 

分 类 号：R917[医药卫生—药物分析学]