胃癌中FOXD3的表达及其与β-catenin表达的相关性  

Expression of FOXD3 in gastric cancer and its correlation withβ-catenin expression

作　　者：邹长棪[1] 林华妹[1] 胡丹[2] 苏颖[1] 夏言 郑雄伟[2,3] 林贤东 ZOU Chang-yan;LIN Hua-mei;HU Dan;SU Ying;XIA Yan;ZHENG Xiong-wei;LIN Xian-dong(Laboratory of Radiation Oncology and Radiobiology,Fujian Cancer Hospital/Fujian Medical University Cancer Hospital,Fuzhou 350014,China;Department of Pathology,Fujian Cancer Hospital/Fujian Medical University Cancer Hospital,Fuzhou 350014,China;Fujian Provincial Key Laboratory of Translational Cancer Medicine,Fuzhou 350014,China)

机构地区：[1]福建省肿瘤医院/福建医科大学附属肿瘤医院放射生物学及肿瘤放射治疗研究室,福州350014 [2]福建省肿瘤医院/福建医科大学附属肿瘤医院病理科,福州350014 [3]福建省肿瘤转化医学重点实验室,福州350014

出　　处：《临床与实验病理学杂志》2020年第2期143-147,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：福建省科技厅面上项目(2019J01196);福建省科技创新联合项目(2017Y9082、2018Y9113);福建省医学创新项目(2019-CX-5)。

摘　　要：目的探讨FOXD3表达与胃癌临床病理学特征、预后及β-catenin表达的相关性。方法收集78例胃癌及癌旁组织,采用qRT-PCR法检测FOXD3 mRNA的表达,采用免疫组化EnVision法检测β-catenin的表达。结果FOXD3 mRNA在胃癌组织中的表达量为0.146±0.036,在癌旁组织中的表达量为-0.379±0.107,上调3.35倍(P=0.001)。FOXD3表达与胃癌分化程度和Lauren分型密切相关(P<0.05)。Kaplan-Meier分析结果显示,FOXD3高表达组总体生存率(overall survival,OS)比低表达组差(P=0.046)。Spearsman等级相关性分析结果显示,胃癌中FOXD3表达与β-catenin表达呈正相关(r=0.280,P=0.023)。结论FOXD3在胃癌组织中过表达并与胃癌组织分化程度、Lauren分型及预后密切相关,FOXD3可能通过Wnt/β-catenin信号促进胃癌的发生、发展,FOXD3可能是判断胃癌预后的分子标志物。Purpose To investigate the relationship between the expression level of FOXD3 in gastric cancer tissues and clinicopathological features,prognosis and expression ofβ-catenin.Methods 78 cases with gastric cancer and adjacent tissue were collected.The mRNA expression level of FOXD3 was detected by qRT-PCR and the expression ofβ-catenin was detected by immunohistochemistry.Results The expression level of FOXD3 in gastric cancer tissues was 0.146±0.036,and the expression level of FOXD3 in gastric paracancerous tissues was-0.379±0.107,which was up-regulated by 3.35 times(P<0.001).The expression of FOXD3 was closely related to the differentiation of gastric cancer and Lauren typing(P<0.05).Kaplan-Meier analysis showed that the overall survival rate of gastric cancer with FOXD3 high expression was lower than that of gastric cancer with FOXD3 with low expression(P=0.046).Spearsman rank correlation analysis showed that FOXD3 expression was positively correlated withβ-catinin expression(r=0.280,P=0.023),and it was not closely related to E-cadherin and vimentin expression.Conclusions FOXD3 is overexpressed in gastric cancer tissues and is closely related to tumor differentiation,Lauren’s type and prognosis.FOXD3 may promote the occurrence and development of gastric cancer through Wnt/β-catenin signaling.FOXD3 may be a molecular marker for judging the prognosis of gastric cancer.

关 键 词：胃肿瘤 FOXD3 Β-CATENIN 预后 信号通路 

分 类 号：R737.9[医药卫生—肿瘤]