原发性肝细胞癌组织中CYP2J2和EETs的表达及临床意义  

Correlation between CYP2J2 and EETs expression in primary hepatocellular carcinoma and its clinical significance

作　　者：徐强[1] 宋蒨[1] 周瑜[1] 徐元丰[1] 巢浩强 石红建[1] XU Qiang;SONG Qian;ZHOU Yu;XU Yuan-feng;CHAO Hao-qiang;SHI Hong-jian(Department of Interventional Vascular Surgery,Changzhou Wujin People’s Hospital,Changzhou 213000,China)

机构地区：[1]江苏大学附属武进人民医院(徐州医科大学武进临床学院)介入血管科,常州213000

出　　处：《临床与实验病理学杂志》2020年第2期154-158,共5页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨原发性肝细胞癌(hepatocellular carcinoma,HCC)中细胞色素P450表氧化酶2J2(cytochrome P450 arachidonic acid epoxygenase 2J2,CYP2J2)和环氧二十碳三烯酸(epoxyeicosatrienoic acids,EETs)的表达及临床意义。方法采用免疫组化SP法检测86例HCC组织中CYP2J2和EETs的表达,分析CYP2J2和EETs表达与HCC临床病理特征及患者预后的相关性。结果CYP2J2高表达率为41.86%(36/86),低表达率为58.14%(50/86);EETs高表达率为52.33%(45/86),低表达率为47.67%(41/86)。CYP2J2和EETs高表达患者更多见于Ⅲ+Ⅳ期、中+低分化、合并静脉癌栓和淋巴结转移的胃癌。Kaplan-Meier生存分析显示,CYP2J2低表达组患者的2年生存率(52.00%,26/50)高于CYP2J2高表达组(30.56%,11/36),差异有统计学意义(χ^2=9.282,P=0.002);EETs低表达组患者的2年生存率(58.54%,24/41)高于EETs高表达组(26.67%,12/45),差异有统计学意义(χ^2=14.969,P=0.009)。多因素Cox回归分析显示,CYP2J2、EETs是影响HCC患者生存时间的危险因素(P<0.05)。经Spearman等级相关检验发现,CYP2J2和EETs表达呈正相关(r=0.810,P<0.001)。结论CYP2J2和EETs表达与HCC肿瘤分期、分化程度、是否合并静脉癌栓和淋巴结转移具有明显相关性;且CYP2J2、EETs为影响HCC患者预后的危险因素。CYP2J2和EETs能为HCC患者的诊疗及预后预测提供参考依据。Purpose To investigate the correlation between the expression of cytochrome P450 arachidonic acid epoxygenase 2J2(CYP2J2)and epoxyeicosatrienoic acids(EETs)in primary hepatocellular carcinoma(HCC)and its clinical significance.Methods Immunohistochemistry was used to detect the expression levels of CYP2J2 and EETs in 86 HCC paraffin-embedded tissues.The correlation between the expression of CYP2J2 and EETs in HCC patients and their clinicopathological features and prognosis were analyzed.Results The high expression rate of CYP2J2 was 41.86%(36/86),and the low expression rate was 58.14%(50/86).The high expression rate of EETs was 52.33%(45/86),and the low expression rate was 47.67%(41/86).Patients with high expression of CYP2J2 and EETs were more likely to have stage III+IV,moderately poorly differentiated,with venous tumor thrombus and lymph node metastasis.Kaplan-Meier survival analysis showed that the 2-year survival rate of patients with low expression of CYP2J2 was 52.00%(26/50 cases),which was higher than that of CYP2J2 high expression group(30.56%,11/36 cases),the difference was statistically significant(χ^2=9.282,P=0.002).The 2-year survival rate of patients with low EETs expression was 58.54%(24/41),which was higher than that of patients with high expression of EETs(26.67%,12/45 cases).The difference was statistically significant(χ^2=14.969,P=0.009).Multivariate cox regression analysis showed that CYP2J2 and EETs were risk factors for survival time of HCC patients(P<0.05).According to the Spearman rank correlation test,the expression of CYP2J2 and EETs was positively correlated(r=0.810,P<0.001).Conclusion The expression status of CYP2J2 and EETs is significantly correlated with the clinicopathological features of HCC.CYP2J2 and EETs are risk factors for the prognosis of patients with HCC.CYP2J2 and EETs can provide reference for the diagnosis and treatment of patients with HCC.

关 键 词：肝肿瘤 原发性肝细胞癌 细胞色素P450表氧化酶2J2 环氧二十碳三烯酸 预后 

分 类 号：R735.7[医药卫生—肿瘤]