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作 者:李晋 张伟[1] 李中武[1] 程杰[1] 杨建荣[1] LI Jin;ZHANG Wei;LI Zhongwu;CHENG Jie;YANG Jianrong(Jiangsu Key Laboratory of Oral Diseases,Department of Oral and Maxillofacial Surgery,Affiliated Stomatological Hospital of Nanjing Medical University,Nanjing 210029,China)
机构地区:[1]南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院口腔颌面外科,江苏南京210029
出 处:《口腔生物医学》2020年第1期36-40,共5页Oral Biomedicine
基 金:国家自然科学基金(81602378);江苏高校优势学科建设工程资助项目(2018-87)。
摘 要:目的:研究TEA转录因子4(TEAD4)在口腔鳞癌(OSCC)组织中的表达与临床病理参数、预后的关系。方法:免疫组化检测79例原发性OSCC标本和21例口腔正常上皮黏膜中TEAD4的表达水平,分析其与临床病理参数之间的关系。使用小干扰RNA(siRNA)转染沉默Cal27细胞内源性TEAD4,通过Western blot、qRT-PCR、划痕试验、Transwell侵袭等实验探究TEAD4沉默对OSCC细胞系Cal27迁移、侵袭的影响。结果:免疫组化结果显示TEAD4在OSCC组织中表达明显高于正常口腔黏膜(P<0.05)。TEAD4高表达与患者颈淋巴结转移、临床分期和患者总体生存率相关(P<0.05)。TEAD4沉默后Cal27细胞侵袭、迁移能力明显减弱。结论:TEAD4高表达与OSCC恶性表型及预后有关,参与OSCC细胞迁移、侵袭表型的调控。Objective:To investigate the expression of TEA domain transcription factor 4(TEAD4)in oral squamous cell carcinoma(OSCC)and its correlation with clinical pathological parameters and prognosis value,and explore the biological function in OSCC invasion and migration ability.Methods:Immunohistochemistry was assessed to detect TEAD4 expression in 79 primary OSCC sample and 21 normal oral mucosa.The associations between TEAD4 and multiple clinicopathological parameters and prognosis were analyzed.Western Blot,qRT-PCR,wound healing assay and Transwell invasion assay were assessed to detect the invasion and migration ability of Cal27 when TEAD4 silencing.Results:Immunohistochemistry data showed that the expression of TEAD4 in OSCC was significantly higher than that in normal oral mucosa(P<0.05).And its expression in OSCC was significantly correlated with cervical lymph node metastasis,clinical stage,and overall survival rate(P<0.05).The invasion and migration ability of Cal27 was impaired when TEAD4 silencing.Conclusions:Our findings reveal that overexpression of TEAD4 correlate with OSCC malignant phenotype,the prognosis of patients and promoting OSCC cells migration and invasion.
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