沉默HOXB7抑制人宫颈癌细胞系CASKI转移与增强顺铂敏感性  

Silencing HOXB7 inhibits metastasis and increasesthe sensitivity to cisplatin in human cervical cancer CASKI cells

作　　者：孙慧霞[1] 郭哲[1] 许静[1] SUN Hui-xia;GUO Zhe;XU Jing(Department of Gynecology, Nanyang City Center Hospital, Nanyang 473000, China)

机构地区：[1]南阳市中心医院妇产科,河南南阳473000

出　　处：《基础医学与临床》2020年第4期532-537,共6页Basic and Clinical Medicine

摘　　要：目的探究HOXB7对人宫颈癌细胞系CASKI迁移、侵袭以及顺铂敏感性的影响。方法用免疫组化法和Western blot分析宫颈癌化疗敏感组织和化疗耐药组织中HOXB7表达。用免疫荧光法和Western blot检测CASKI细胞和宫颈癌顺铂耐药CASKI/DDP细胞中HOXB7表达。sh-HOXB7转染CASKI细胞,用Transwell小室法分析细胞的侵袭和迁移能力,用CCK-8法和流式细胞术检测细胞对顺铂敏感性的影响。结果宫颈癌化疗耐药组织以及CASKI/DDP细胞中HOXB7高表达(P<0.001);沉默HOXB7抑制CASKI细胞的迁移与侵袭能力(P<0.001),促进CASKI细胞对顺铂敏感性(P<0.001)。结论沉默HOXB7抑制宫颈癌CASKI细胞的转移并促进细胞对顺铂的敏感性。Objective To explore the effect of HOXB7 on migration,invasion and the sensitivity to cisplatin in cervical cancer CASKI cells.Methods The expression level of HOXB7 in chemotherapy sensitive tissues and chemotherapy resistant tissues was detected by immunohistochemistry and Western blot.The expression level of HOXB7 in CASKI cells and cisplatin-resistant CASKI/DDP cells was detected by immunofluorescence and Western blot.sh-HOXB7 was transfected into CASKI cells and the cell invasion and migration were analyzed by transwell analysis,the sensitivity to cisplatin was detected by CCK-8 assay and flow cytometry.ResultsHigh expression of HOXB7 was found in chemoresistant tissues and CASKI/DDP cells(P<0.001);Silencing HOXB7 inhibited migration and invasion(P<0.001)and increased the sensitivity to cisplatin in CASKI cells(P<0.001).Conclusions Silencing HOXB7 inhibits metastasis and increases the sensitivity to cisplatin in cervical cancer CASKI cells.

关 键 词：HOXB7 宫颈癌 侵袭 迁移 顺铂耐药 

分 类 号：R73-37[医药卫生—肿瘤] R73-35+4[医药卫生—临床医学]