丙硫菌唑对斑马鱼的安全性评价及其生物富集行为研究  

Study on Biotoxicity and Bioaccumulation of Prothioconazole in Brachydanio rerio

作　　者：郭宝元 张洋[1] 王松雪 Guo Baoyuan;Zhang Yang;Wang Songxue(Academy of National Food and Strategic Reserves Administration,Beijing 100037,China;Key Laboratory of Environmental Biotechnology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China)

机构地区：[1]国家粮食和物质储备局科学研究院,北京100037 [2]中国科学院生态环境研究中心,中国科学院环境生物技术重点实验室,北京100085

出　　处：《生态毒理学报》2019年第6期311-316,共6页Asian Journal of Ecotoxicology

基　　金：“十三五”国家科技重大专项(2017ZX07107);北京市科技计划课题项目(Z181100005418010)。

摘　　要：丙硫菌唑是一种市场前景非常好的新型广谱杀菌剂。本文研究了丙硫菌唑对水生生物斑马鱼的急性毒性和生物累积风险。通过斑马鱼的急性毒性试验获得丙硫菌唑对斑马鱼的96 h-LC50为2.06 mg a.i.·L-1。随后,采用0.02 mg·L-1(1/100 LC50)和0.2 mg·L-1(1/10 LC50)2个浓度的丙硫菌唑,通过8 d实验,获得其在斑马鱼体内的生物累积效应。在0.02 mg·L-1组中,第8天时,斑马鱼体内的浓度达到0.733 mg·kg-1,生物富集系数(BCF8 d)缓慢增长到34.36。而在0.2 mg·L-1组,第8天时,斑马鱼组织内丙硫菌唑浓度为4.198 mg·kg-1, BCF8 d值为19.72。结果表明,丙硫菌唑对斑马鱼的毒性等级为中毒,同时其在斑马鱼体内具有中等生物累积效应。因此,在使用过程中,需要考虑其对水生生物的毒性和水生食物链的放大效应。Prothioconazole is a new broad-spectrum fungicide and of good application prospects. To evaluate the environmental risk, the biotoxicity and bioaccumulation in zebrafish(Brachydanio rerio) were studied. The 96 hLC50 to zebrafish was 2.06 mg a.i.·L-1 by acute toxicity test. Accordingly, the concentrations of 0.02 mg·L-1(1/100 LC50) and 0.2 mg·L-1(1/10 LC50) were employed to evaluate the biological availability of prothioconazole in Brachydanio rerio. Under the exposure level of 0.02 mg·L-1, the concentration of prothioconazole in zebrafish increased rapidly in the first day and moderately later. The concentration was 0.733 mg·kg-1 in zebrafish on the 8 th day and bioaccumulation factor(BCF8 d) was 34.36. Under the level of 0.2 mg·L-1, the concentration of prothioconazole in zebrafish increased in the first few days and reached equilibrium in 96 h. The concentration was 4.198 mg·kg-1 in zebrafish and BCF8 dwas 19.72. The results showed that prothioconazole was of moderate toxicity and of medium bioconcentration in zebrafish. Therefore, the toxicity, bioaccumulation and magnification of prothioconazole in food chain should be considered during the application.

关 键 词：丙硫菌唑 斑马鱼 急性毒性 生物累积 

分 类 号：X171.5[环境科学与工程—环境科学]