芍药甘草汤主要成分在正常及多囊卵巢综合征大鼠尿液和粪便中的代谢产物分析  

Analysis on Metabolites of Major Components of Shaoyao Gancaotang in Urine and Feces of Normal and Polycystic Ovary Syndrome Rats

作　　者：郭一婷 程瑶 邵云云[2] 刘俊瑾 冯小娟 张京平[2] 侯锐刚[2] GUO Yi-ting;CHENG Yao;SHAO Yun-yun;LIU Jun-jin;FENG Xiao-juan;ZHANG Jing-ping;HOU Rui-gang(Shanxi Medical University,Taiyuan 030000,China;Second Hospital of Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]山西医科大学,太原030000 [2]山西医科大学第二医院,太原030001

出　　处：《中国实验方剂学杂志》2020年第6期103-112,共10页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省中药现代化关键技术研究振东专项(2014ZD0102);山西省卫生计生委科研课题(2015046)。

摘　　要：目的:分析及鉴定芍药甘草汤主要成分在正常及多囊卵巢综合征(PCOS)大鼠尿液和粪便中的代谢产物,探究PCOS对芍药甘草汤代谢的影响。方法:雌性SD大鼠随机分为正常组和PCOS组,利用来曲唑溶液灌胃21 d制备PCOS大鼠模型,观察动情周期;采用UHPLC-QTRAP-MS/MS技术并结合Light Sight 2. 3软件对芍药甘草汤主要成分在正常及PCOS大鼠尿液和粪便中的代谢产物进行分析与鉴定,流动相水-甲醇梯度洗脱,流速0. 3 m L·min-1,电喷雾离子源,负离子模式。结果:药物代谢主要发生Ⅰ相及Ⅱ相反应,正常组大鼠尿液中共检测到代谢物27个,PCOS组大鼠尿液中共检测到代谢物34个;正常组大鼠粪便样品中共检测到代谢物29个,PCOS组大鼠粪便样品中共检测到代谢物27个。结论:PCOS大鼠尿液中代谢产物比正常大鼠更具多样性,PCOS疾病状态可能会影响芍药甘草汤有效成分在体内的代谢途径。Objective: To analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and polycystic ovary syndrome( PCOS) rats,and to explore the effect of PCOS on the metabolism of Shaoyao Gancaotang. Method: Female SD rats were randomly divided into normal group and PCOS group. PCOS rat model was prepared by administration of letrozole solution for 21 days,and the estrous cycle of rats was observed. UHPLC-QTRAP-MS/MS technique and LightSight 2. 3 software were used to analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and PCOS rats. The mobile phase was water-methanol for gradient elution,the flow rate was 0. 3 m L·min-1,electrospray ion source was employed under negative ion mode. Result: Phase Ⅰ and phase Ⅱ reactions mainly occurred in drug metabolism. A total of 27 metabolites were detected in urine of normal rats,and 34 metabolites were detected in urine of PCOS rats. A total of 29 metabolites were detected in feces of normal rats,and 27 metabolites were detected in feces of PCOS rats. Conclusion: The metabolites in the urine of PCOS rats are more diverse than those of normal rats,and the disease status of PCOS may affect the in vivo metabolic pathway of active ingredients in Shaoyao Gancaotang.

关 键 词：多囊卵巢综合征 芍药甘草汤 代谢产物 代谢途径 尿液 粪便 肠道菌群 

分 类 号：R22[医药卫生—中医基础理论] R969[医药卫生—中医学]