聚乙烯醇生物降解菌群的结构分析及优势菌株降解特性  

Analysis of microbial community structure of poly(vinyl acohol) degradation and the degradation characteristics of the dominant strain

作　　者：陈萍 王晨媛 贾智锐 荆硕 张毅[1] CHEN Ping;WANG Chenyuan;JIA Zhirui;JING Shuo;ZHANG Yi(School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China)

机构地区：[1]华南理工大学生物科学与工程学院,广州510006

出　　处：《环境工程学报》2020年第2期560-568,共9页Chinese Journal of Environmental Engineering

基　　金：广东自然科学基金资助项目(2013B090600028)。

摘　　要：对堆肥中降解聚乙烯醇材料的微生物菌群结构进行了分析。结果表明:降解聚乙烯醇材料的优势菌群属于芽胞杆菌科(Bacillaceae)。从降解了3年的材料表面筛选出了1株聚乙烯醇降解菌DG01,鉴定为苏云金杆菌(Bacillus thuringiensis sp.)。分别以聚乙烯醇(poly(vinyl alcohol), PVA)浓度和二氧化碳排放量为指标,对PVA的降解动力学进行了研究。结果表明:PVA生物降解过程符合一级动力学模型,R2分别为0.984 0和0.983 5。对摇瓶培养条件进行了单因素优化实验。最佳降解温度,初始pH和酵母粉浓度分别为41℃、7和1.40 g·L^-1。优化后,48 h内PVA初始浓度为3 g·L^-1的降解率达到了45.21%,提高了2.10倍。Microbial community structure of degrading ploy(vinyl alcohol) material during the compost burial course was analyzed. The results showed that Bacillaceae was the dominant bacteria in the microbial community for ploy(vinyl alcohol) material degradation. A PVA degrading bacterial strain DG01 was isolated from PVA materials surface after three-year degradation, and was identified as Bacillus thuringiensis sp. Taking PVA concentration of and CO2 emission as indicators, the PVA degradation kinetics was explored. Results showed that PVA degradation process fitted well with the pseudo-first-order kinetic model. The correlation coefficients R2 were 0.984 0 and 0.983 5, respectively. PVA degradation conditions were optimized with single-factor experimental design under shaking flask culture. The optimum conditions for PVA biodegradation were 41 ℃,initial pH 7 and yeast concentration of 1.40 g·L^-1. For PVA with 3 g·L^-1 initial concentration, the corresponding degradation rate reached 45.21% within the first 48 h, which increased by 2.10 times.

关 键 词：聚乙烯醇 菌群结构分析 苏云金杆菌 生物降解 

分 类 号：X172[环境科学与工程—环境科学]