转染DCN基因对COPD大鼠成纤维细胞(TGF-β1/Smads)信号通路的影响  

Effect of Transfected DCN Gene on TGF-β1/Smads Signaling Pathway in COPD Rat Fibroblasts

作　　者：杨兰 周洵 郭坤坤 葛正行 刘明慧 李改改 YANG Lan;ZHOU Xun;GUO Kunkun;GE Zhengxing;LIU Minghui;LI Gaigai(Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550002;Second Affiliated Hospital of Guizhou University of Chinese Medicine,Guiyang,Guizhou 550001;Xinle Hospital of Traditional Chinese Medicine,Xinle,Hebei 050700)

机构地区：[1]贵州中医药大学,贵州贵阳550002 [2]贵州中医药大学第二附属医院,贵州贵阳550001 [3]新乐市中医医院,河北新乐050700

出　　处：《贵州中医药大学学报》2020年第2期27-34,共8页Journal of Guizhou University of Traditional Chinese Medicine

基　　金：贵州省科技厅立项资助,项目编号:黔科合SY字(2013)3071号;贵州省科技合作计划项目,项目编号:黔科合LH字[2015]7258号;贵州省中医药管理局中医药、民族医药科学技术研究专项课题,项目编号:QZYY-2015-017;贵州省科技合作计划项目,项目编号:黔科合J重大字[2015]2002。

摘　　要：目的:通过探究转染DCN基因对成纤维细胞TGF-β1、Smad3、Smad7表达及细胞增殖的影响,探讨DCN基因对TGF-β1/Smads信号通路的调控作用机制。方法:采用Real time PCR检测TGF-β1、Smad3和Smad7基因的表达变化。结果:通过对转染后的成纤维细胞进行Real time PCR检测、细胞增殖检测:转染组与对照组相比,DCNmRNA、Smad7mRNA表达量明显增加,而TGF-β1mRNA、Smad3mRNA表达量明显减少,差别均有统计学意义(P<0.05)。结论:通过转染DCN基因对COPD大鼠气道成纤维细胞影响的实验观测(TGF-β1、Smad3和Smad7)基因的表达变化,转染DCN基因对TGF-β1/Smads信号通路产生抑制作用,干预成纤维细胞增殖速度。Objective:To explore the regulatory mechanism of DCN gene expressed on TGF-beta 1,Smad3,Smad7 and cell proliferation in fibroblasts by investigating the effect of transfected DCN gene on TGF-beta 1/Smads signaling pathway.Methods:The real time PCR was used to detected the expression changes of TGF-beta 1,Smad3 and Smad7 genes.Results:The transfected fibroblasts were detected by Real time PCR and cell proliferation:compared with the control group,the expressions of DCNmRNA and Smad7mRNA were significantly increased in the transfection group,while the expressions of TGF-beta 1mRNA and Smad3mRNA were significantly decreased in the transfection group,with statistically significant differences(P<0.05).Conclusion:Through the experimental observation of the influence of transfected DCN gene on airway fibroblasts of COPD rats(TGF-beta 1,Smad3 and Smad7 gene expression changes,the transfected DCN gene inhibited TGF-beta 1/Smads signaling pathway and interfered with the proliferation rate of fibroblasts.

关 键 词：信号通路 DCN TGF-β1 Smad3 SMAD7 慢性阻塞性肺病 

分 类 号：R363[医药卫生—病理学] R373[医药卫生—基础医学]