彗星电泳检测草胺磷对蚯蚓体腔细胞DNA的损伤  

Detection of DNA Damage in Eisenia fetida Coelomocytes Induced by Glufosinate with Comet Assay

作　　者：张来军 陈永敢 王凤琴[2] ZHANG Laijun;CHEN Yonggan;WANG Fengqin(College of Fisheries and Life Science,Hainan Tropical Ocean University/Hainan Key Laboratory of Herpetological Research,Sanya 572022,China;College of Life Sciences and Technology,Longdong University,Qingyang 745000,China)

机构地区：[1]海南热带海洋学院水产与生命学院/海南省两栖爬行动物研究重点实验室,海南三亚572022 [2]陇东学院生命科学与技术学院,甘肃庆阳745000

出　　处：《广东农业科学》2020年第1期87-91,共5页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31260139)。

摘　　要：【目的】探讨草胺磷对土壤动物的影响,以蚯蚓为受试动物,在实验室环境下,采用彗星电泳技术检测不同浓度草胺磷胁迫对蚯蚓体腔细胞DNA损伤的程度。【方法】草胺磷质量浓度设定为0、37.5、75、150、300 mg/L,胁迫时间24 h,取头部DNA含量、尾部DNA含量、尾长、尾矩、Olive尾矩5个参数分析DNA损伤程度。【结果】电泳结果显示,与空白对照比较,5个参数在4个草胺磷处理中都有显著差异;随草胺磷浓度升高,尾部DNA含量、尾长、尾矩、Olive尾矩随之升高,头部DNA含量随之降低,草胺磷胁迫引起了蚯蚓体腔细胞DNA的损伤,草胺磷浓度越高,DNA损伤越严重。随后降低草胺磷浓度,延长胁迫时间至96 h,得到相似结果。【结论】草胺磷会引起土壤动物蚯蚓体腔细胞DNA的损伤,草胺磷浓度与蚯蚓体腔细胞DNA损伤程度两者间存在显著的剂量-效应关系。【Objective】In order to investigate the effect of glufosinate on the soil animals,Eisenia fetida was selected as tested animal and the comet assay was used to detect DNA damage of E.fetida coelomocytes under the stress of glufosinate with different concentrations.【Method】Under laboratory conditions,the concentrations of glufosinate were set at 0,37.5,75,150 and 300mg/L,and stress time as 24h.The degree of DNA damage was expressed by five parameters,i.e.HDNA content,TDNA content,tail length,tail moment and Olive tail moment.【Result】There were significant differences in five parameters between four treatment groups and the control group.With the increase of glufosinate concentration,TDNA content,tail length,tail moment and Olive tail moment increased,while HDNA content decreased.DNA damage of coelomocytes was caused by glufosinate,and the higher the concentration of glufosinate was,the more serious the DNA damage was.Then,the concentration of glufosinate was reduced stress time extended to 96h,and the same results were obtained.【Conclusion】Glufosinate caused DNA damage to coelomocytes of E.fetida.There was a significant dose-effect relationship between the concentration of glufosinate and the DNA damage of E.fetida coelomocytes.

关 键 词：草胺磷 赤子爱胜蚓 细胞毒性 DNA损伤 体腔细胞 彗星电泳 

分 类 号：X171.5[环境科学与工程—环境科学]