Comparative Transcriptome Analysis between Cytoplasmic Male-sterile Line and Its Maintainer During the Floral Bud Development of Pepper  

作　　者：Junheng Lv Zhoubin Liu Yuhua Liu Lijun Ou Minghua Deng JingWang Jingshuang Song Yanqing Ma Wenchao Chen Zhuqing Zhang Xiongze Dai Xuexiao Zou 

机构地区：[1]Longping Branch,Graduate School of Hunan University,Changsha 410125,China [2]Vegetable Institution of Hunan Academy of Agricultural Sciences,Changsha 410125,China [3]College of Landscape and Horticulture,Yunnan Agricultural University,Kunming 650201,China

出　　处：《Horticultural Plant Journal》2020年第2期89-98,共10页园艺学报（英文版）

基　　金：This work was supported by China Agriculture Research System(Grant No.CARS-23-G29).

摘　　要：The male-sterile line has been largely used in the hybrid seed production of pepper, which can effectively improve the efficiency of hybrid seed production. However, the formation mechanism of male sterility in pepper remains unclear. In the present study, we compared the gene expression patterns between pepper cytoplasmic male sterile line 9704 A and its maintainer 9704 B during floral bud development using RNA sequencing technology. A total of 547 976 and 2 416 Differentially Expressed Genes(DEGs) were identified in the stage S1, S2 and S3, respectively,and more than 70% of the DEGs were down-regulated in the sterile line. Gene Ontology(GO), and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses were performed to further understand the functions of these identified DEGs. The results showed that the DEGs were mainly enriched in pathways of starch and sucrose metabolism, pentose and glucuronate interconversions. A number of genes, such as MS1, PME5, ATPB, and lots of transcription factors were found down-regulated in the sterile line, and we also identified a series of genes with large differences in expression patterns between sterile line and maintainer line. Collectively, our findings laid a foundation for further molecular breeding in pepper and provided new insights into its mechanism underlying the male sterility.

关 键 词：PEPPER CAPSICUM annuum CYTOPLASMIC male STERILITY COMPARATIVE transcriptome analysis DEGs 

分 类 号：S641.3[农业科学—蔬菜学]